Research Strand Single Molecules

Research Strand:  Single-molecule approaches to understand complex, dynamic biological processes

vignette single molecule

Using state-of-the-art single molecule microscopes developed within LISCB, we are investigating complex and dynamic biological processes including the initiation of transcription (Revyakin and Panne) and the assembly of splicing complexes (Eperon and Hudson). These approaches allow us to address questions that are beyond the reach of other methods. This includes understanding the stoichiometry of protein assemblies and importantly, the nature of the dynamic processes through which large biological complexes are assembled. These approaches complement the static views provided by other structural biology approaches.