Professor IC Eperon
Professor of Biochemistry
School/Department: Molecular Cell Biology, Department of
Telephone: +44 (0)116 229 7012
Email: eci@leicester.ac.uk
Profile
Research
We have adopted a multi-disciplinary approach to this very challenging question, based on a combination of single molecule total internal reflection fluorescence microscopy with novel chemical biology tools. This is facilitated by a BBSRC sLoLa award that supports a consortium including colleagues in Leicester, Glasgow and Strathclyde with expertise in optics, structural biology, bio-organic chemistry and nano-engineering..
Publications
(0) Eperon, I.C., Anderson, S. and Nierlich, D.P. (1980). Distinctive sequence of human mitochondrial ribosomal RNA genes. Nature 286, 460-467.
Anderson, S., Bankier, A. T., Barrell, B. G., de Bruijn, M. H., Coulson, A. R., Drouin, J., Eperon, I. C., Nierlich, D. P., Roe, B. A., Sanger, F., Schreier, P. H., Smith, A. J., Staden, R., and Young, I. G.(1981). The sequence and organisation of the human mitochondrial genome. Nature 290, 457-467.
Eperon, L.P., Estibeiro, J.P. and Eperon, I.C. (1986). The role of nucleotide sequences in splice site selection in eukaryotic pre-messenger RNA. Nature 324, 280-282.
Eperon, L.P., Graham, I.R., Griffiths, A.D. and Eperon, I.C. (1988). The effects of RNA secondary structure on alternative splicing of pre-mRNA: is folding limited to a region behind the transcribing RNA polymerase? Cell 54, 393-401.
Eperon, I.C., Ireland, D.C., Smith, R.A., Mayeda, A., and Krainer, A.R. (1993). Pathways for selection of 5' splice sites by U1 snRNPs and SF2/ASF. EMBO J. 12, 3607-3617.
Venables, J.P., Elliott, D.J., Makarova, O.V., Makarov, E.M., Cooke, H.J,.& Eperon, I.C. (2000). RBMY, a probable human spermatogenesis factor, and other hnRNP G proteins interact with Tra2b and affect splicing. Hum. Mol. Gen. 9, 685-694.
Eperon, I.C., Makarova, O., Mayeda, A., Munroe, S.H., Cáceres, J., Hayward, D.G., & Krainer, A.R. (2000) Selection of alternative 5' splice sites: the role of U1 snRNP and models for the antagonistic affects of SF2/ASF and hnRNP A1. Mol. Cell. Biol. 20, 8303-8318.
Skordis, L.A., Dunckley, M.G., Yue, B.-G., Eperon, I.C., and Muntoni, F. (2003). Bifunctional antisense oligonucleotides provide a trans-acting splicing enhancer that stimulates SMN2 gene expression in patient fibroblasts. Proc. Natl Acad. Sci. USA 100, 4114-4119.
Cherny, D., Gooding, C., Eperon, G.E., Coelho, M.B., Bagshaw, C.R., Smith, C.W.J., & Eperon, I.C. (2010). Stoichiometry of a regulatory splicing complex revealed by single molecule analyses. EMBO J. 29, 2161-2172.
Weldon, C., Behm-Ansmant, I., Hurley, L.H., Burley, G.A., Branlant, C., Eperon, I.C., and Dominguez, C. (2017). Identification of G-quadruplexes in functional RNAs using 7-deaza-guanine. Nature Chemical Biology 13, 18-20.
Jobbins, A.M., Reichenbach, L.F., Lucas, C.M., Hudson, A.J., Burley, G.A., & Eperon, I.C. (2018). The mechanisms of a mammalian splicing enhancer. Nucleic Acids Research 46, 2145-2158.
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Supervision
Teaching
BS2091: From genes to proteins