Meet our team

The CBS facilities works closely with one another, sharing their extensive range of skills and expertise, to ensure they deliver the most appropriate solutions to researcher’s key scientific questions.

Core Biological Services Manager

Ila Patel

ip14@le.ac.uk
+44 (0)116 252 5277


Advanced Imaging Facility

Expertise

  • Bright field and DIC
  • Large area imaging /slide scanning 
  • 2D/3D/4D imaging
  • Fluorescence 
  • Spectral imaging 
  • Bioluminescence imaging
  • F-techniques (FRAP, FLIP, FRET and FCS)
  • Correlative Light Electron Microscopy (CLEM)
  • High content screening
  • Image processing and analysis
  • TIRF

Equipment

  • FujifilmLAS4000
  • Leica TCS SP5 confocal laser scanning microscope
  • Nikon C1Si confocal laser scanning microscope
  • Olympus FV1000 confocal laser scanning microscope
  • VisiTech Infinity3 confocal laser microscope
  • Zeiss multiphoton laser scanning microscope
  • Nikon TE300 wide field microscope
  • Nikon Ti-E inverted Microscope 1
  • Nikon Ti-E inverted microscope 2
  • Olympus BX61 fluorescence and spectral microscope
  • Olympus CellR/ScanR live cell imaging and screening station
  • Bioluminescence Imaging System LV200
  • Typhoon Trio Plus, Imager and Scanner

Contact

Kees Straatman
krs5@le.ac.uk
+44 (0)116 229 7085 
+44 (0)116 252 2263


Bioinformatics Analysis Support Hub (BASH)

Expertise

  • Bioinformatics assistance 
  • Data analysis
  • Workshops and courses

Equipment

  • Access to ALICE - University of Leicester high performance computing cluster 
  • NGS user database

Contact

Jin-Li Luo
jl19@le.ac.uk
+44 (0)116 252 3350


Containment Level 3 Facility

Expertise

  • Hazard group 3 microorganisms
  • Routine bacteriology, molecular biology, tissue culture and small rodent models of infection
  • Assistance for HSE and Home Office documentation, experimental procedure design and risk assessment, animal studies

Equipment

  • 4 laboratories
  • ACDP Cat III
  • CO2 incubators

Contact

C3Facility@le.ac.uk


Electron Microscopy Facility

Expertise

  • TEM Imaging
  • SEM Imaging
  • TEM Prep – Resin Embedding, Immunogold labelling, Ultramicrotomy, Tomography
  • TEM Prep - Negative staining, Glow discharge/plasma cleaning
  • Correlative Light Electron Microscopy (CLEM)
  • SEM Prep - Critical point drying, sputter coating, Volume electron microscopy (SBFSEM) - sample preparation and consultation
  • Human, animal and plant tissues
  • Cell cultures and cell fractions
  • Micro-organisms, bacteria, biofilms and viruses
  • Membrane vesicles, exosomes and microparticles
  • Proteins and macromolecules
  • Single particle analysis
  • Nanoparticles and drug delivery mechanisms

Equipment

  • JEOl JEM-1400 TEM
  • Hitachi S3000H SEM
  • Quorum Q150 TES
  • Balzers Critical Point Dryer
  • Leica Ultracut UC7
  • Reichert Ultracut E

Contact

Natalie Allcock
nsa2@le.ac.uk
+44 (0)116 252 3370


Flow Cytometry Facility

Expertise

  • Assay Support, Panel Design and Data Analysis
  • Multi colour assay
  • Assay transfer, development and validation.
  • Flowjo Software

Equipment

  • BD Facscanto II
  • FACSAria Fusion
  • FACSanto II
  • Cytoflex
  • Accuri C6 plus

Contact

Ania Straatman-Iwanowska 
FlowCytometry@leicester.ac.uk
+44 (0)116 229 7502


NUCLEUS Genomics

Expertise

  • Single cell Transcriptomics
  • qPCR
  • Liquid Handling
  • Fluorospectrometry
  • Genome sequencing
  • Microarray

Equipment

  • ABI 7900HT PCR
  • Lightcycler 480 Real Time PCR
  • Beckman Coulter UK- Biomek FX System
  • NanoDrop 3300 Fluorospectrometer
  • Roche GS FLX genome sequencer
  • Illumina Bead Station 500
  • Illumina iScan array scanner
  • Miseq Desktop Sequencer

Contact

Nic Sylvius
Research Associate
ns249@le.ac.uk
+44 (0)116 252 3365

Reshma Vaghela
Experimental Officer
rjb40@le.ac.uk
+44 (0)116 252 3365


Histology Facility

Expertise

  • Collection and preparation of human and animal tissue
  • Cutting of frozen tissue
  • Cutting and staining to include Haematoxylin & Eosin, range of special stains (Perl`s, PAS, etc.)
  • Fixation and processing
  • Immunohistochemistry

Equipment

  • Leica CM1950
  • Leica EG1160
  • Afos VST 1 RH Ventilated Specimen Table
  • Leica ST4040 Linear H&E Stainer
  • Leica RM2235 rotary microtome
  • Microtome 2125RT
  • Leica ASP300 tissue processor

Contact

Angie Gillies
akg8@le.ac.uk
+44 (0)116 252 3235


Protex

Expertise

  • Vector Design, modification
  • High through-put cloning including mutagenesis
  • Protein expression and purification
  • SCRISPR-Cas9 genome editing

Contact

Ila Patel
ip14@le.ac.uk
+44 (0)116 2525277 


X-Ray Irradiation facility

Expertise

  • Safe irradiation of in vitro and in vivo biological samples within a laboratory environment.
  • Safer, simpler and less costly alternative to radioisotope irradiators for radiation exposure studies.

Equipment

  • RS320 X–Ray Research System

Contact 

Natalie Allcock
X-rayIrradiator@leicester.ac.uk