Meet our team
CBS facilities combine their extensive range of skills and expertise to ensure appropriate solutions to researcher’s key scientific questions.
Core Biological Services Manager
Dr Sarah Glenn
smg28@le.ac.uk
+44 (0)116 252 2992
Advanced Imaging Facility
Expertise
- Bright field and DIC
- Large area imaging /slide scanning
- 2D/3D/4D imaging
- Fluorescence
- Spectral imaging
- F-techniques (FRAP, FLIP, FRET and FCS)
- Correlative Light Electron Microscopy (CLEM)
- Super-resolution imaging
- High content screening
- Image processing and analysis
- TIRF
Equipment
- Zeiss LSM980 Airyscan2 confocal laser scanning microscope
- Nikon C1Si confocal laser scanning microscope
- Olympus FV1000 confocal laser scanning microscope
- VisiTech Infinity3 confocal laser microscope
- Nikon Ti-E inverted Microscope 1
- Nikon Ti-E inverted microscope 2
- Olympus BX61 fluorescence and spectral microscope
- Olympus CellR/ScanR live cell imaging and screening station
- Phasefocus Livecyte 2 label free imaging system
- Akoya PhenoImager slide scanner
- Akoya PhenoCycler-Fusion imaging platform
Contact
Dr Kees Straatman
krs5@le.ac.uk
+44 (0)116 229 7085
+44 (0)116 252 2263
- See more about the Advanced Imaging Facility
Containment Level 3 Facility
Expertise
- Hazard group 3 microorganisms
- Routine bacteriology, molecular biology, tissue culture and small rodent models of infection
- Assistance for HSE and Home Office documentation, experimental procedure design and risk assessment, animal studies
Equipment
- 4 laboratories
- ACDP Cat III
- CO2 incubators
Contact
- See more about the Containment Level 3 Facility
Electron Microscopy Facility
Expertise
- TEM Imaging
- SEM Imaging
- TEM Prep – Resin Embedding, Immunogold labelling, Ultramicrotomy, Tomography
- TEM Prep - Negative staining, Glow discharge/plasma cleaning
- Correlative Light Electron Microscopy (CLEM)
- SEM Prep - Critical point drying, sputter coating, Volume electron microscopy (SBFSEM) - sample preparation and consultation
- Human, animal and plant tissues
- Cell cultures and cell fractions
- Micro-organisms, bacteria, biofilms and viruses
- Membrane vesicles, exosomes and microparticles
- Proteins and macromolecules
- Single particle analysis
- Nanoparticles and drug delivery mechanisms
Equipment
- JEOl JEM-1400 TEM
- Zeiss Gemini 360 FEGSEM
- Quorum Q150 TES
- Balzers Critical Point Dryer
- Leica Ultracut UC7
- Reichert Ultracut E
Contact
Natalie Allcock
nsa2@le.ac.uk
+44 (0)116 252 3370
- See more about the Electron Microscopy Facility
Flow Cytometry Facility
Expertise
- Assay Support, Panel Design and Data Analysis
- Multi colour assay
- Assay transfer, development and validation.
- Flowjo Software
Equipment
- BD Facscanto II
- FACSAria Fusion
- FACSanto II
- Cytoflex
- Accuri C6 plus
Contact
Reshma Vaghela
FlowCytometry@leicester.ac.uk
+44 (0)116 229 7502
- See more about the Flow Cytometry Facility
NUCLEUS Genomics
Expertise
- Single cell Transcriptomics
- qPCR
- Liquid Handling
- Fluorospectrometry
- Genome sequencing
- Microarray
Equipment
- ABI 7900HT PCR
- Lightcycler 480 Real Time PCR
- Beckman Coulter UK- Biomek FX System
- NanoDrop 3300 Fluorospectrometer
- Roche GS FLX genome sequencer
- Illumina Bead Station 500
- Illumina iScan array scanner
- Miseq Desktop Sequencer
Contact
Dr Nic Sylvius
Research Associate
ns249@le.ac.uk
+44 (0)116 252 3365
- See more about the NUCLEUS Genomics
Histology Facility
Expertise
- Collection and preparation of human and animal tissue
- Cutting of frozen tissue
- Cutting and staining to include Haematoxylin & Eosin, range of special stains (Perl`s, PAS, etc.)
- Fixation and processing
- Immunohistochemistry
Equipment
- Leica CM1950
- Leica EG1160
- Afos VST 1 RH Ventilated Specimen Table
- Leica ST4040 Linear H&E Stainer
- Leica RM2235 rotary microtome
- Microtome 2125RT
- Leica ASP300 tissue processor
Contact
Janine Moreton
histologylab@le.ac.uk
+44 (0)116 252 3235
- See more about the Histology Facility
Protex
Expertise
- Vector Design, modification
- High through-put cloning including mutagenesis
- Protein expression and purification
- SCRISPR-Cas9 genome editing
Contact
Sharon Munday
slm75@le.ac.uk
+44 (0)116 252 7089
X-Ray Irradiation facility
Expertise
- Safe irradiation of in vitro and in vivo biological samples within a laboratory environment.
- Safer, simpler and less costly alternative to radioisotope irradiators for radiation exposure studies.
Equipment
- RS320 X–Ray Research System
Contact
Natalie Allcock
X-rayIrradiator@leicester.ac.uk
- See more about the X-Ray Irradiation facility