Core Biotechnology Services

Meet our team

CBS facilities combine their extensive range of skills and expertise to ensure appropriate solutions to researcher’s key scientific questions.

Core Biological Services Manager 

Dr Sarah Glenn

smg28@le.ac.uk
+44 (0)116 252 2992


Advanced Imaging Facility

Expertise

  • Fluorescence, bright field, DIC and label free imaging
  • Widefield, super-resolution and confocal imaging
  • Spectral imaging 
  • 2D/3D/4D imaging
  • High content screening
  • Large area imaging/whole slide scanning 
  • F-techniques (FRAP, FLIP, FRET)
  • Correlative Light Electron Microscopy (CLEM)
  • Image processing and analysis
  • TIRF

Equipment

  • Zeiss LSM980 Airyscan2 confocal laser scanning microscope
  • Zeiss Elyra 7 lattice SIM2 with STORM and TIRF
  • Nikon C1Si confocal laser scanning microscope
  • Olympus FV1000 confocal laser scanning microscope
  • VisiTech Infinity3 confocal laser microscope
  • Nikon Ti-E inverted Microscope 1
  • Nikon Ti-E inverted microscope 2
  • Olympus BX61 fluorescence and spectral microscope
  • Olympus CellR/ScanR live cell imaging and screening station
  • Phasefocus Livecyte 2 label free imaging system
  • Akoya PhenoImager slide scanner
  • Akoya PhenoCycler-Fusion imaging platform

Contact

Dr Kees Straatman
krs5@le.ac.uk
+44 (0)116 229 7085 


Containment Level 3 Facility

Expertise

  • Hazard group 3 microorganisms
  • Routine bacteriology, molecular biology, tissue culture and small rodent models of infection
  • Assistance for HSE and Home Office documentation, experimental procedure design and risk assessment, animal studies

Equipment

  • 4 laboratories
  • ACDP Cat III
  • CO2 incubators

Contact

C3Facility@le.ac.uk


Electron Microscopy Facility

Expertise

  • TEM Imaging
  • SEM Imaging
  • TEM Prep – Resin Embedding, Immunogold labelling, Ultramicrotomy, Tomography
  • TEM Prep - Negative staining, Glow discharge/plasma cleaning
  • Correlative Light Electron Microscopy (CLEM)
  • SEM Prep - Critical point drying, sputter coating, Volume electron microscopy (SBFSEM) - sample preparation and consultation
  • Human, animal and plant tissues
  • Cell cultures and cell fractions
  • Micro-organisms, bacteria, biofilms and viruses
  • Membrane vesicles, exosomes and microparticles
  • Proteins and macromolecules
  • Single particle analysis
  • Nanoparticles and drug delivery mechanisms 

Equipment

  • JEOl JEM-1400 TEM
  • Zeiss Gemini 360 FEGSEM
  • Quorum Q150 TES
  • Balzers Critical Point Dryer
  • Leica Ultracut UC7
  • Reichert Ultracut E

Contact

Natalie Allcock
nsa2@le.ac.uk
+44 (0)116 252 3370


Flow Cytometry Facility

Expertise

  • Assay Support, Panel Design and Data Analysis
  • Multi colour assay
  • Assay transfer, development and validation.
  • Flowjo Software

Equipment

  • BD Facscanto II
  • FACSAria Fusion
  • FACSanto II
  • Cytoflex
  • Accuri C6 plus

Contact

Reshma Vaghela 
FlowCytometry@leicester.ac.uk
+44 (0)116 229 7502


NUCLEUS Genomics

Expertise

  • Single cell Transcriptomics
  • qPCR
  • Liquid Handling
  • Fluorospectrometry
  • Genome sequencing
  • Microarray

Equipment

  • ABI 7900HT PCR
  • Lightcycler 480 Real Time PCR
  • Beckman Coulter UK- Biomek FX System
  • NanoDrop 3300 Fluorospectrometer
  • Roche GS FLX genome sequencer
  • Illumina Bead Station 500
  • Illumina iScan array scanner
  • Miseq Desktop Sequencer

Contact

Dr Nic Sylvius
Research Associate
ns249@le.ac.uk
+44 (0)116 252 3365


Histology Facility

Expertise

  • Collection and preparation of human and animal tissue
  • Cutting of frozen tissue
  • Cutting and staining to include Haematoxylin & Eosin, range of special stains (Perl`s, PAS, etc.)
  • Fixation and processing
  • Immunohistochemistry

Equipment

  • Leica CM1950
  • Leica EG1160
  • Afos VST 1 RH Ventilated Specimen Table
  • Leica ST4040 Linear H&E Stainer
  • Leica RM2235 rotary microtome
  • Microtome 2125RT
  • Leica ASP300 tissue processor

Contact

Janine Moreton
histologylab@le.ac.uk
+44 (0)116 252 3235


Protex

Expertise

  • Vector Design, modification
  • High through-put cloning including mutagenesis
  • Protein expression and purification
  • SCRISPR-Cas9 genome editing

Contact

Sharon Munday
slm75@le.ac.uk
+44 (0)116 252 7089 


X-Ray Irradiation facility

Expertise

  • Safe irradiation of in vitro and in vivo biological samples within a laboratory environment.
  • Safer, simpler and less costly alternative to radioisotope irradiators for radiation exposure studies.

Equipment

  • RS320 X–Ray Research System

Contact 

Natalie Allcock
X-rayIrradiator@leicester.ac.uk

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