Core Biotechnology Services
Meet our team
CBS facilities combine their extensive range of skills and expertise to ensure appropriate solutions to researcher’s key scientific questions.
Core Biological Services Manager
Dr Sarah Glenn
smg28@le.ac.uk
+44 (0)116 252 2992
Advanced Imaging Facility
Expertise
- Fluorescence, bright field, DIC and label free imaging
- Widefield, super-resolution and confocal imaging
- Spectral imaging
- 2D/3D/4D imaging
- High content screening
- Large area imaging/whole slide scanning
- F-techniques (FRAP, FLIP, FRET)
- Correlative Light Electron Microscopy (CLEM)
- Image processing and analysis
- TIRF
Equipment
- Zeiss LSM980 Airyscan2 confocal laser scanning microscope
- Zeiss Elyra 7 lattice SIM2 with STORM and TIRF
- Nikon C1Si confocal laser scanning microscope
- Olympus FV1000 confocal laser scanning microscope
- VisiTech Infinity3 confocal laser microscope
- Nikon Ti-E inverted Microscope 1
- Nikon Ti-E inverted microscope 2
- Olympus BX61 fluorescence and spectral microscope
- Olympus CellR/ScanR live cell imaging and screening station
- Phasefocus Livecyte 2 label free imaging system
- Akoya PhenoImager slide scanner
- Akoya PhenoCycler-Fusion imaging platform
Contact
Dr Kees Straatman
krs5@le.ac.uk
+44 (0)116 229 7085
- See more about the Advanced Imaging Facility
Containment Level 3 Facility
Expertise
- Hazard group 3 microorganisms
- Routine bacteriology, molecular biology, tissue culture and small rodent models of infection
- Assistance for HSE and Home Office documentation, experimental procedure design and risk assessment, animal studies
Equipment
- 4 laboratories
- ACDP Cat III
- CO2 incubators
Contact
- See more about the Containment Level 3 Facility
Electron Microscopy Facility
Expertise
- TEM Imaging
- SEM Imaging
- TEM Prep – Resin Embedding, Immunogold labelling, Ultramicrotomy, Tomography
- TEM Prep - Negative staining, Glow discharge/plasma cleaning
- Correlative Light Electron Microscopy (CLEM)
- SEM Prep - Critical point drying, sputter coating, Volume electron microscopy (SBFSEM) - sample preparation and consultation
- Human, animal and plant tissues
- Cell cultures and cell fractions
- Micro-organisms, bacteria, biofilms and viruses
- Membrane vesicles, exosomes and microparticles
- Proteins and macromolecules
- Single particle analysis
- Nanoparticles and drug delivery mechanisms
Equipment
- JEOl JEM-1400 TEM
- Zeiss Gemini 360 FEGSEM
- Quorum Q150 TES
- Balzers Critical Point Dryer
- Leica Ultracut UC7
- Reichert Ultracut E
Contact
Natalie Allcock
nsa2@le.ac.uk
+44 (0)116 252 3370
- See more about the Electron Microscopy Facility
Flow Cytometry Facility
Expertise
- Assay Support, Panel Design and Data Analysis
- Multi colour assay
- Assay transfer, development and validation.
- Flowjo Software
Equipment
- BD Facscanto II
- FACSAria Fusion
- FACSanto II
- Cytoflex
- Accuri C6 plus
Contact
Reshma Vaghela
FlowCytometry@leicester.ac.uk
+44 (0)116 229 7502
- See more about the Flow Cytometry Facility
NUCLEUS Genomics
Expertise
- Single cell Transcriptomics
- qPCR
- Liquid Handling
- Fluorospectrometry
- Genome sequencing
- Microarray
Equipment
- ABI 7900HT PCR
- Lightcycler 480 Real Time PCR
- Beckman Coulter UK- Biomek FX System
- NanoDrop 3300 Fluorospectrometer
- Roche GS FLX genome sequencer
- Illumina Bead Station 500
- Illumina iScan array scanner
- Miseq Desktop Sequencer
Contact
Dr Nic Sylvius
Research Associate
ns249@le.ac.uk
+44 (0)116 252 3365
- See more about the NUCLEUS Genomics
Histology Facility
Expertise
- Collection and preparation of human and animal tissue
- Cutting of frozen tissue
- Cutting and staining to include Haematoxylin & Eosin, range of special stains (Perl`s, PAS, etc.)
- Fixation and processing
- Immunohistochemistry
Equipment
- Leica CM1950
- Leica EG1160
- Afos VST 1 RH Ventilated Specimen Table
- Leica ST4040 Linear H&E Stainer
- Leica RM2235 rotary microtome
- Microtome 2125RT
- Leica ASP300 tissue processor
Contact
Janine Moreton
histologylab@le.ac.uk
+44 (0)116 252 3235
- See more about the Histology Facility
Protex
Expertise
- Vector Design, modification
- High through-put cloning including mutagenesis
- Protein expression and purification
- SCRISPR-Cas9 genome editing
Contact
Sharon Munday
slm75@le.ac.uk
+44 (0)116 252 7089
X-Ray Irradiation facility
Expertise
- Safe irradiation of in vitro and in vivo biological samples within a laboratory environment.
- Safer, simpler and less costly alternative to radioisotope irradiators for radiation exposure studies.
Equipment
- RS320 X–Ray Research System
Contact
Natalie Allcock
X-rayIrradiator@leicester.ac.uk
- See more about the X-Ray Irradiation facility