Providing a comprehensive range of histological techniques. The facility includes a cut-up area, processor/embedder, cryostat and microtomes. Cutting is generally undertaken on standard blocks but megablocks can also be cut. Haematoxylin and Eosin staining is performed on a linear stainer ensuring consistent results as assessed by the NEQAS quality assurance scheme. Immunohistochemistry can be performed using a variety of techniques to fit the researcher's needs.

Project logistics should first be discussed with the lead technician.

Services

Receipt and sample preparation

• Fresh tissue for freezing and nitrogen storage
• Tissue fixed in 10% Formalin for processing to paraffin wax
• Production of cytoblocks from provided cells

Cutting facility

• Frozen sections of fresh tissue for RNA/DNA extraction (placed into eppendorfs with lysis buffer)
• Frozen sections of fresh tissue on treated slides for staining or Immunohistochemistry
• Wax sections on untreated slides for staining or RNA/DNA extraction
• Wax sections on treated slides for Immunohistochemistry
• Megablock sections on large slides for hand staining

Staining currently available

• Haematoxylin and Eosin
• Immunohistochemistry
• Special stains (e.g. Perls, Elastic-Van Gieson)

Access diagnostic archives on your behalf

Where to find us

Leicester Royal Infirmary 
RKCSB
Level 3
Infirmary Square
Leicester
LE1 5WW

Contact

• Janine Moreton (Histology Manager), +44 (0)116 252 3235