Providing a comprehensive range of histological techniques. The facility includes a cut-up area, processor/embedder, cryostat and microtomes. Cutting is generally undertaken on standard blocks but megablocks can also be cut. Haematoxylin and Eosin staining is performed on a linear stainer ensuring consistent results as assessed by the NEQAS quality assurance scheme. Immunohistochemistry can be performed using a variety of techniques to fit the researcher's needs.
Project logistics should first be discussed with the lead technician.
Services
Receipt and sample preparation
- Fresh tissue for freezing and nitrogen storage
- Tissue fixed in 10% Formalin for processing to paraffin wax
- Production of cytoblocks from provided cells
Cutting facility
- Frozen sections of fresh tissue for RNA/DNA extraction (placed into eppendorfs with lysis buffer)
- Frozen sections of fresh tissue on treated slides for staining or Immunohistochemistry
- Wax sections on untreated slides for staining or RNA/DNA extraction
- Wax sections on treated slides for Immunohistochemistry
- Megablock sections on large slides for hand staining
Staining currently available
- Haematoxylin and Eosin
- Immunohistochemistry
- Special stains (e.g. Perls, Elastic-Van Gieson)
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Where to find us
Leicester Royal Infirmary
RKCSB
Level 3
Infirmary Square
Leicester
LE1 5WW
Contact
- Janine Moreton (Histology Manager), +44 (0)116 252 3235