Histology Facility

Providing a comprehensive range of histological techniques. The facility includes a cut-up area, processor/embedder, cryostat and microtomes. Cutting is generally undertaken on standard blocks but megablocks can also be cut. Haematoxylin and Eosin staining is performed on a linear stainer ensuring consistent results as assessed by the NEQAS quality assurance scheme. Immunohistochemistry can be performed using a variety of techniques to fit the researcher's needs.

Project logistics should first be discussed with the lead technician.

Haematoxylin and Eosin on human spleenServices

Receipt and sample preparation

  • Fresh tissue for freezing and nitrogen storage
  • Tissue fixed in 10% Formalin for processing to paraffin wax
  • Production of cytoblocks from provided cells

Cutting facility

  • Frozen sections of fresh tissue for RNA/DNA extraction (placed into eppendorfs with lysis buffer)
  • Frozen sections of fresh tissue on treated slides for staining or Immunohistochemistry
  • Wax sections on untreated slides for staining or RNA/DNA extraction
  • Wax sections on treated slides for Immunohistochemistry
  • Megablock sections on large slides for hand staining

Staining currently available

  • Haematoxylin and Eosin
  • Immunohistochemistry
  • Special stains (e.g. Perls, Elastic-Van Gieson)

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Where to find us

Leicester Royal Infirmary 
RKCSB
Level 3
Infirmary Square
Leicester
LE1 5WW 

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