Flow Cytometry Facility
Flow Cytometry is a laser-based technology which allows in depth analysis of the characteristics of cells or particles. It is predominantly used to measure fluorescently labelled cells, for example cell populations labelled with fluorescently tagged antibodies, or cells expressing fluorescent proteins. The technique allows the analysis of multiple fluorescent markers on individual cell populations and, when combined with cell sorting capability, allows the selection of key populations of interest which can then undergo further analysis.
Flow Cytometry can be used in a range of applications which include immunophenotyping, single cell sorting for cloning or genomics analysis, cell cycle and cell death assays. However it can be used for any application in which fluorescence or light scatter from particles in suspension can further your research goals.
The core facility at the University of Leicester delivers a state of the art flow cytometry service to support both internal and external collaborative research. The existing facilities have been brought together with three new instruments to form a core facility managed by Jenny Hincks, who brings 15 years' analytical and cell sorting experience to the University.
The facility is situated in lab G43, Maurice Shock Building, and houses a BD FACSAria Fusion cell sorter and three analysers – a BD FACSCanto II, a Beckman Coulter Cytoflex and a BD Accuri C6 Plus. The facility is open for use for all researchers at University of Leicester and for collaborations with external parties.