Core Biotechnology Services



Analysis of Selection Methods to Develop Novel Phage Therapy Cocktails Against Antimicrobial Resistant Clinical Isolates of Bacteria.
Haines MEK, Hodges FE, Nale JY, Mahony J, van Sinderen D, Kaczorowska J, Alrashid B, Akter M, Brown N, Sauvageau D, Sicheritz-Pontén T, Thanki AM, Millard AD, Galyov EE, Clokie MRJ. Front Microbiol. 2021 Mar 29;12:613529. doi: 10.3389/fmicb.2021.613529. eCollection 2021.PMID: 33868187


Genome Sequence and Characterization of Coliphage Vb Eco Slur29.
Besler, Ibrahim, Pavelas Sazinas, Christian Harrison, Lucy Gannon, Tamsin Redgwell, Slawomir Michniewski, Steven P. Hooton, Jon L. Hobman, and Andrew Millard. Phage 1, no. 1 (2020): 38-44.

A Novel Role for Kynurenine 3-Monooxygenase in Mitochondrial Dynamics.
Maddison, D. C., M. Alfonso-Nunez, A. M. Swaih, C. Breda, S. Campesan, N. Allcock, A. Straatman-Iwanowska, C. P. Kyriacou, and F. Giorgini. PLoS Genet 16, no. 11 (Nov 2020)

Focal Epilepsy Modulates Vesicular Positioning at Cortical Synapses.
Vannini, Eleonora, Laura Restani, Marialaura Dilillo, Liam McDonnell, Matteo Caleo, and Vincenzo Marra. Preprint - BioRxiv(2020). 


Dedicated Setup for the Photoconversion of Fluorescent Dyes for Functional Electron Microscopy.
Dobson, Katharine L., Carmel L. Howe, Yuri Nishimura, and Vincenzo Marra. Frontiers in Cellular Neuroscience 13 (2019). 

In Vitro Evaluation of the Inhibitory Effect of Topical Ophthalmic Agents on Acanthamoeba Viability.
Heaselgrave, W., A. Hamad, S. Coles, and S. Hau. Transl Vis Sci Technol 8, no. 5 (Sep 2019): 17. 

vB_PaeM_MIJ3, a Novel Jumbo Phage Infecting Pseudomonas aeruginosa, Possesses Unusual Genomic Features.
Imam M, Alrashid B, Patel F, Dowah ASA, Brown N, Millard A, Clokie MRJ, Galyov EE.Front Microbiol. 2019 Nov 28;10:2772. doi: 10.3389/fmicb.2019.02772. eCollection 2019. PMID: 31849908

Mycobacterial Phosphatase Pstp Regulates Global Serine Threonine Phosphorylation and Cell Division. 
Iswahyudi, G. V. Mukamolova, A. A. Straatman-Iwanowska, N. Allcock, P. Ajuh, O. Turapov, and H. M. O'Hare. Sci Rep 9, no. 1 (Jun 6 2019): 8337. 

The Lytic Transglycosylase, Ltgg, Controls Cell Morphology and Virulence in Burkholderia Pseudomallei.
Jenkins, C. H., R. Wallis, N. Allcock, K. B. Barnes, M. I. Richards, J. M. Auty, E. E. Galyov, S. V. Harding, and G. V. Mukamolova. Sci Rep 9, no. 1 (Jul 30 2019): 11060.

Riding the Wave of Genomics to Investigate Aquatic Coliphage Diversity and Activity.
Michniewski, S., T. Redgwell, A. Grigonyte, B. Rihtman, M. Aguilo-Ferretjans, J. Christie-Oleza, E. Jameson, D. J. Scanlan, and A. D. Millard. Environ Microbiol 21, no. 6 (Jun 2019): 2112-28.

Functional Assessment of Microbial Superoxide Dismutase Isozymes Suggests a Differential Role for Each Isozyme.
Najmuldeen, H., R. Alghamdi, F. Alghofaili, and H. Yesilkaya. Free Radic Biol Med 134 (Apr 2019): 215-28.

Optimisation of the Preservation Conditions for Molecularly Imprinted Polymer Nanoparticles Specific for Trypsin.
Safaryan, Abeer H. M., Adam M. Smith, Thomas S. Bedwell, Elena V. Piletska, Francesco Canfarotta, and Sergey A. Piletsky. Nanoscale Advances (2019).

Genomic Characterization of Jumbo Salmonella Phages That Effectively Target United Kingdom Pig-Associated Salmonella Serotypes.
Thanki, Anisha M., Nathan Brown, Andrew D. Millard, and Martha R. J. Clokie. Frontiers in Microbiology 10 (2019). 


Ex Vivo Modelling of the Formation of Inflammatory Platelet-Leucocyte Aggregates and Their Adhesion on Endothelial Cells, an Early Event in Sepsis.
Alharbi, Azzah, Jonathan P Thompson, Nicholas P Brindle, and Cordula Stover. Clinical and Experimental Medicine (2018).

Ferritag Is a New Genetically-Encoded Inducible Tag for Correlative Light-Electron Microscopy.
Clarke, N. I. and S. J. Royle. Nat Commun 9, no. 1 (Jul 4 2018): 2604. 

Intracellular Replication of Streptococcus Pneumoniae inside Splenic Macrophages Serves as a Reservoir for Septicaemia.
Ercoli, G., V. E. Fernandes, W. Y. Chung, J. J. Wanford, S. Thomson, C. D. Bayliss, K. Straatman, P. R. Crocker, A. Dennison, L. Martinez-Pomares, P. W. Andrew, E. R. Moxon, and M. R. Oggioni. Nat Microbiol 3, no. 5 (May 2018): 600-10. 

Molecularly Imprinted Polymer Nanoparticle-Based Assay (Mina): Application for Fumonisin B1 Determination.
Munawar, H., K. Smolinska-Kempisty, A. G. Cruz, F. Canfarotta, E. Piletska, K. Karim, and S. A. Piletsky. Analyst 143, no. 14 (Jul 9 2018): 3481-88.

Two Faces of Cwlm, an Essential Pknb Substrate, in Mycobacterium Tuberculosis.
Turapov, O., F. Forti, B. Kadhim, D. Ghisotti, J. Sassine, A. Straatman-Iwanowska, A. R. Bottrill, P. J. Moynihan, R. Wallis, P. Barthe, M. Cohen-Gonsaud, P. Ajuh, W. Vollmer, and G. V. Mukamolova. Cell Rep 25, no. 1 (Oct 2 2018): 57-67 e5. 

Characterisation of Crispr Mutants Targeting Genes Modulating Pectin Degradation in Ripening Tomato.
Wang, D., N. Samsulrizal, C. Yan, N. S. Allcock, J. Craigon, B. Blanco-Ulate, I. Ortega-Salazar, S. E. Marcus, H. M. Bagheri, L. Perez-Fons, P. D. Fraser, T. Foster, R. G. Fray, J. P. Knox, and G. B. Seymour. Plant Physiol (Nov 20 2018).


Biology and Genomics of an Historic Therapeutic Escherichia coli Bacteriophage Collection.
Baig A, Colom J, Barrow P, Schouler C, Moodley A, Lavigne R, Atterbury R. Front Microbiol. 2017 Aug 30;8:1652. doi: 10.3389/fmicb.2017.01652. eCollection 2017. PMID: 28912765

Suppressors of the Chloroplast Protein Import Mutant tic40 Reveal a Genetic Link between Protein Import and Thylakoid Biogenesis.
Bédard J, Trösch R, Wu F, Ling Q, Flores-Pérez Ú, Töpel M, Nawaz F, Jarvis P.Plant Cell. 2017 Jul;29(7):1726-1747. doi: 10.1105/tpc.16.00962. Epub 2017 Jul 6.PMID: 28684427

Air pollution alters Staphylococcus aureus and Streptococcus pneumoniae biofilms, antibiotic tolerance and colonisation.
Hussey SJK, Purves J,Allcock N, Fernandes VE, Monks PS, Ketley JM, Andrew PW, Morrissey JA. Environ Microbiol. 2017 May;19(5):1868-1880. doi: 10.1111/1462-2920.13686. Epub 2017 Feb 28.PMID: 28195384

Microencapsulation of Clostridium Difficile Specific Bacteriophages Using Microfluidic Glass Capillary Devices for Colon Delivery Using Ph Triggered Release. Vinner, G. K., G. T. Vladisavljevic, M. R. J. Clokie, and D. J. Malik. PLoS One 12, no. 10 (2017): e0186239.

New Tools for "Hot-Wiring" Clathrin-Mediated Endocytosis with Temporal and Spatial Precision. Wood, LA, G Larocque, N Clarke, S Sarkar, and SJ Royle. J Cell Biol. 2017 Dec 4;216(12):4351-4365. doi: 10.1083/jcb.201702188. Epub 2017 Sep 27. PMID: 28954824; PMCID: PMC5716275.


Formation of Target-Specific Binding Sites in Enzymes: Solid-Phase Molecular Imprinting of Hrp. Czulak, J., A. Guerreiro, K. Metran, F. Canfarotta, A. Goddard, R. H. Cowan, A. W. Trochimczuk, and S. Piletsky.  Nanoscale 8, no. 21 (Jun 07 2016): 11060-6. 

Occurrence of Primary Lymphocytic Hypophysitis in Two Horses and Presence of Scattered T-Lymphocytes in the Normal Equine Pituitary Gland. Grau-Roma, L., R. Peckham, J. Paton, A. Stahel, and S. de Brot.  J Vet Diagn Invest (Nov 15 2016).

Salad Leaf Juices Enhance Salmonella Growth, Fresh Produce Colonisation and Virulence. Koukkidis, G., R. Haigh, N. Allcock, S. Jordan, and P. Freestone.  Appl Environ Microbiol (Nov 18 2016). 

Bidirectional Counterregulation of Human Lung Mast Cell and Airway Smooth Muscle Beta2 Adrenoceptors. Lewis, R. J., L. Chachi, C. Newby, Y. Amrani, and P. Bradding.  J Immunol 196, no. 1 (Jan 1 2016): 55-63.

'Get in Early’; Biofilm and Wax Moth (Galleria Mellonella) Models Reveal New Insights into the Therapeutic Potential of Clostridium Difficile Bacteriophages. Nale, Janet Y., Mahananda Chutia, Philippa Carr, Peter T. Hickenbotham, and Martha R. J. Clokie. Frontiers in Microbiology 7 (2016).

Bacteriophage Combinations Significantly Reduce Clostridium Difficile Growth in Vitro and Proliferation in Vivo. Nale, J. Y., J. Spencer, K. R. Hargreaves, A. M. Buckley, P. Trzepinski, G. R. Douce, and M. R. Clokie.  Antimicrob Agents Chemother 60, no. 2 (Feb 2016): 968-81.

Detachment of Surface Membrane Invagination Systems by Cationic Amphiphilic Drugs. Osman, S., K. A. Taylor, N. Allcock, R. D. Rainbow, and M. P. Mahaut-Smith.  Sci Rep 6 (2016): 18536.

Intracellular Localisation Ofmycobacterium Marinumin Mast Cells. Siad, Sadiyo, Simon Byrne, Galina Mukamolova, and Cordula Stover.  World Journal of Immunology 6, no. 1 (2016): 83.

Two Novel Myoviruses from North of Iraq Reveal Insights into C.Diff Phage Diversity and Biology. SJ, Rashid, Barylski J, K. R. Hargreaves, Millard AA, Vinner GK, and J J P Clokie.  Viruses 8, no. 310 (2016).


Hyperphosphatemia, Phosphoprotein Phosphatases, and Microparticle Release in Vascular Endothelial Cells. Abbasian, N., J. O. Burton, K. E. Herbert, B. E. Tregunna, J. R. Brown, M. Ghaderi-Najafabadi, N. J. Brunskill, A. H. Goodall, and A. Bevington.  J Am Soc Nephrol 26, no. 9 (Sep 2015): 2152-62.

Rab11 Modulates Alpha-Synuclein-Mediated Defects in Synaptic Transmission and Behaviour. Breda, C., M. L. Nugent, J. G. Estranero, C. P. Kyriacou, T. F. Outeiro, J. R. Steinert, and F. Giorgini.  Hum Mol Genet 24, no. 4 (Feb 15 2015): 1077-91.

Premyelinated Central Axons Express Neurotoxic Nmda Receptors: Relevance to Early Developing White-Matter Injury. Huria, T., N. M. Beeraka, B. Al-Ghamdi, and R. Fern.  J Cereb Blood Flow Metab 35, no. 4 (Apr 2015): 543-53.

Sialic Acid Supplementation Ameliorates Puromycin Aminonucleoside Nephrosis in Rats. Pawluczyk, I. Z., M. G. Najafabadi, J. R. Brown, A. Bevington, and P. S. Topham.  Lab Invest 95, no. 9 (Sep 2015): 1019-28.

Bacteriophage Delivery by Nebulization and Efficacy against Phenotypically Diverse Pseudomonas Aeruginosa from Cystic Fibrosis Patients.
Sahota, J. S., C. M. Smith, P. Radhakrishnan, C. Winstanley, M. Goderdzishvili, N. Chanishvili, A. Kadioglu, C. O'Callaghan, and M. R. Clokie.  J Aerosol Med Pulm Drug Deliv 28, no. 5 (Oct 2015): 353-60.

Genetic and Physical Interaction Studies Reveal Functional Similarities between Albino3 and Albino4 in Arabidopsis
Trosch, R., M. Topel, U. Flores-Perez, and P. Jarvis.  Plant Physiol 169, no. 2 (Oct 2015): 1292-306.

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