Department of Molecular and Cell Biology
E coli
E. coli
* E. coli DE3 hosts: MUST express T7 RNA polymerase
* You MUST contact Dr. Julie Morrissey at the Department of Genetics to sign an MTA agreement before using pLEICS-151
| Vector name | AR | Affinity Tag | Protease Cleavage | Family |
|---|---|---|---|---|
| pLEICS-01 | Amp | N-HIS6 | TEV Extra SM | C |
| pLEICS-02 | Amp | N-GST | TEV Extra SM | C |
| pLEICS-03 | Kan | N-HIS6 | TEV Extra SM | C |
| pLEICS-04 | Kan | N-GST | TEV Extra SM | C |
| pLEICS-05 | Amp | C-HIS6 | TEV Extra ENLYFQ | B |
| pLEICS-06 | Amp | N-HIS6/C-HIS6/S-Tag | TEV Extra ENLYFQ | B/C |
| pLEICS-07 | Kan | N-HIS6/S-Tag | TEV Extra GA | A |
| pLEICS-08 | Kan | N-GST/N-HIS6/S-Tag | TEV Extra GA | A |
| pLEICS-09 | Amp | N-Trx/N-HIS6/S-Tag | TEV Extra GA | A |
| pLEICS-10 | Amp | N-MBP | TEV Extra GA | A |
| pLEICS-14 | Amp | N-GST | TEV Extra GA | A |
| pLEICS-17 | Kan | N-GST-His6 | TEV Extra GA | A |
| pLEICS-18 | Kan | N-MBP | TEV Extra GA | A |
| pLEICS-43 | Amp | N-HIS6-MISTIC | TEV Extra SM | C |
| pLEICS-44 | Kan | N-HIS6-MISTIC | TEV Extra SM | C |
| pLEICS-45 | Amp | N-HIS6/mEGFP | TEV Extra GA | J/K |
| PLEICS-46 | Kan | N-GB1/HIS6 | TEV Extra GA | A |
| pLEICS-50 | Amp | C-GST | TEV Extra ENLYFQ | B |
| pLEICS-51 | Kan | N-HIS6-N-FlAsH | TEV Extra SM | C |
| pLEICS-52 | Kan | N-HIS6-N-FlAsH/mEGFP128 | TEV Extra SM | C |
| pLEICS-53 | Kan | N-HIS6-N-FlAsH/mEGFP129 | TEV Extra SM | C |
| pLEICS-54 | Kan | N-HIS6/N-mEGFP128 | TEV Extra SM | C |
| pLEICS-55 | Kan | N-HIS6/N-mEGFP129 | TEV Extra SM | C |
| pLEICS-56 | Kan | Super-mEGFP1-215 | TEV Extra SM | C |
| pLEICS-57 | Kan | N-HIS6/Super-mEGFP216-232 | TEV Extra SM | C |
| pLEICS-58 | Amp | mEGFP128 | TEV Extra SM | C |
| pLEICS-59 | Amp | mEGFP129 | TEV Extra SM | C |
| pLEICS-60 | Kan | N-HIS6-FlAsH Super-mEGFP1-215 | TEV Extra SM | C |
| pLEICS-61 | Amp | N-HIS6-FlAsH | TEV Extra SM | C |
| pLEICS-63 | Amp | C-Super folded mEGFP-HIS6 | TEV Extra ENLYFQ | B |
| pLEICS-64 | Amp | C-mEGFP-HIS6 | TEV Extra ENLYFQ | B |
| pLEICS-65 | Amp | C-Super folded mEGFP1-10/HIS6 | TEV Extra ENLYFQ | B |
| pLEICS-66 | Amp | C-Super folded mEGFP11/HIS6 | TEV Extra ENLYFQ | B |
| pLEICS-67 | Amp | N-HIS6/mCherry | TEV Extra GA | J/K |
| pLEICS-68 | Amp | N-HIS6/mEYFP | TEV Extra GA | J/K |
| pLEICS-69 | Amp | N-GST | PreScission Protease Extra GP | 69 |
| pLEICS-89 | Amp | N-His6/S/TEV-Halo | TEV Extra GA | 89 |
| pLEICS-90 | Amp | C-Halo-TEV-HIS6 | TEV Extra ENLYFQ | 90 |
| pLEICS-91 | Amp | N-GB1/HIS6 | TEV Extra GA | A |
| pLEICS-92 | Amp | N-GST-His6 | TEV Extra GA | A |
| pLEICS-93 | Amp | N-HIS6/S-Tag | TEV Extra GA | A |
| pLEICS-151 | Tet | Vector for complementation inPseudomonas aeuriginosa | 151 |