E coli

E. coli

* E. coli DE3 hosts: MUST express T7 RNA polymerase
* You MUST contact Dr. Julie Morrissey at the Department of Genetics to sign an MTA agreement before using pLEICS-151

Vector name AR Affinity Tag Protease Cleavage Family
pLEICS-01 Amp N-HIS6 TEV Extra SM C
pLEICS-02 Amp N-GST TEV Extra SM C
pLEICS-03 Kan N-HIS6 TEV Extra SM C
pLEICS-04 Kan N-GST TEV Extra SM C
pLEICS-05 Amp C-HIS6 TEV Extra ENLYFQ B
pLEICS-06 Amp N-HIS6/C-HIS6/S-Tag TEV Extra ENLYFQ B/C
pLEICS-07 Kan N-HIS6/S-Tag TEV Extra GA A
pLEICS-08 Kan N-GST/N-HIS6/S-Tag TEV Extra GA A
pLEICS-09 Amp N-Trx/N-HIS6/S-Tag TEV Extra GA A
pLEICS-10 Amp N-MBP TEV Extra GA A
pLEICS-14 Amp N-GST TEV Extra GA A
pLEICS-17 Kan N-GST-His6 TEV Extra GA A
pLEICS-18 Kan N-MBP TEV Extra GA A
pLEICS-43 Amp N-HIS6-MISTIC TEV Extra SM C
pLEICS-44 Kan N-HIS6-MISTIC TEV Extra SM C
pLEICS-45 Amp N-HIS6/mEGFP TEV Extra GA J/K
PLEICS-46 Kan N-GB1/HIS6 TEV Extra GA A
pLEICS-50 Amp C-GST TEV Extra ENLYFQ B
pLEICS-51 Kan N-HIS6-N-FlAsH TEV Extra SM C
pLEICS-52 Kan N-HIS6-N-FlAsH/mEGFP128 TEV Extra SM C
pLEICS-53 Kan N-HIS6-N-FlAsH/mEGFP129 TEV Extra SM C
pLEICS-54 Kan N-HIS6/N-mEGFP128 TEV Extra SM C
pLEICS-55 Kan N-HIS6/N-mEGFP129 TEV Extra SM C
pLEICS-56 Kan Super-mEGFP1-215 TEV Extra SM C
pLEICS-57 Kan N-HIS6/Super-mEGFP216-232 TEV Extra SM C
pLEICS-58 Amp mEGFP128 TEV Extra SM C
pLEICS-59 Amp mEGFP129 TEV Extra SM C
pLEICS-60 Kan N-HIS6-FlAsH Super-mEGFP1-215 TEV Extra SM C
pLEICS-61 Amp N-HIS6-FlAsH TEV Extra SM C
pLEICS-63 Amp C-Super folded mEGFP-HIS6 TEV Extra ENLYFQ B
pLEICS-64 Amp C-mEGFP-HIS6 TEV Extra ENLYFQ B
pLEICS-65 Amp C-Super folded mEGFP1-10/HIS6 TEV Extra ENLYFQ B
pLEICS-66 Amp C-Super folded mEGFP11/HIS6 TEV Extra ENLYFQ B
pLEICS-67 Amp N-HIS6/mCherry TEV Extra GA J/K
pLEICS-68 Amp N-HIS6/mEYFP TEV Extra GA J/K
pLEICS-69 Amp N-GST PreScission Protease Extra GP 69
pLEICS-89 Amp N-His6/S/TEV-Halo TEV Extra GA 89
pLEICS-90 Amp C-Halo-TEV-HIS6 TEV Extra ENLYFQ 90
pLEICS-91 Amp N-GB1/HIS6 TEV Extra GA A
pLEICS-92 Amp N-GST-His6 TEV Extra GA A
pLEICS-93 Amp N-HIS6/S-Tag TEV Extra GA A
pLEICS-151 Tet Vector for complementation inPseudomonas aeuriginosa 151