Department of Molecular and Cell Biology
CRISPR
CRISPR vectors
Vector Name | AR | Selection | CRISPR Effector | gRNA and Cleavage | PAM Sequence | Host | Family |
---|---|---|---|---|---|---|---|
pLEICS-95 | Amp | EGFP | Cas9 | All-in-one, Nick (D10A) | 3'-NGG | Mammalian cells | 95 |
pLEICS-96 | Amp | Puromycin | Cas9 | All-in-one, Nick (D10A) | 3'-NGG | Mammalian cells | 95 |
pLEICS-97 | Amp | Puromycin | Cas9 | All-in-one, Cut | 3'-NGG | Mammalian cells | 95 |
pLEICS-98 | Kan | Cas9 | gRNA | Zebrafish | 98 | ||
pLEICS-99 | Amp | mCherry | Cas9 | All-in-one, Nick (D10A) | 3'-NGG | Mammalian cells | 95 |
pLEICS-100 | Amp | mCherry | Cas9 | All-in-one, Cut | 3'-NGG | Mammalian cells | 95 |
pLEICS-101 | Amp | mEGFP | Cas9 | All-in-one, Cut | 3'-NGG | Mammalian cells | 95 |
pLEICS-102 | Amp | Neomycin | Cas9 | All-in-one, Nick (D10A) | 3'-NGG | Mammalian cells | 95 |
pLEICS-103 | Amp | Neomycin | Cas9 | All-in-one, Cut | 3'-NGG | Mammalian cells | 95 |
pLEICS-104 | Amp | Zeocin | Cas9 | All-in-one, Nick (D10A) | 3'-NGG | Mammalian cells | 95 |
pLEICS-105 | Amp | Zeocin | Cas9 | All-in-one, Cut | 3'-NGG | Mammalian cells | 95 |
pLEICS-106 | Amp | Puromycin | Cas9 | All-in-one, Cut | 3'-NGG | Lentivirus | 95 |
pLEICS-107 | Amp | Puromycin | Cas9 | gRNA | Lentivirus | 95 | |
pLEICS-108 | Amp | Blasticidin | Cas9 | Cas9 | 3'-NGG | Lentivirus | |
pLEICS-109 | Amp | Puromycin | Cas9 | All-in-one, Inducible Cas9, Cut | 3'-NGG | Lentivirus | 95 |
pLEICS-110 | Amp | Puromycin | Cas9 | All-in-one, Inducible Cas9, Cut | 3'-NGG | Mammalian cells | 95 |
pLEICS-111 | Amp | Neomycin | Cas9 | All-in-one, Inducible Cas9, Cut | 3'-NGG | Mammalian cells | 95 |
pLEICS-112 | Amp | Puromycin | Cas9 | All-in-one, Nick (D10A) | 3'-NGAG | Mammalian cells | 95 |
pLEICS-113 | Amp | Puromycin | Cas9 | All-in-one, Cut | 3'-NGAG | Mammalian cells | 95 |
pLEICS-114 | Amp | mCherry | Cas9 | All-in-one, Nick (D10A) | 3'-NGAG | Mammalian cells | 95 |
pLEICS-115 | Amp | mCherry | Cas9 | All-in-one, Cut | 3'-NGAG | Mammalian cells | 95 |
pLEICS-116 | Amp | Neomycin | Cas9 | All-in-one, Nick (D10A) | 3'-NGAG | Mammalian cells | 95 |
pLEICS-117 | Amp | Neomycin | Cas9 | All-in-one, Cut | 3'-NGAG | Mammalian cells | 95 |
pLEICS-118 | Amp | Puromycin | Cpf1 | Staggered Cut | 5'-TTN | Mammalian cells | 118 |
pLEICS-119 | Amp | Neomycin | Cpf1 | Staggered Cut | 5'-TTN | Mammalian cells | 118 |
pLEICS-120 | Amp | mEGFP | Cpf1 | Staggered Cut | 5'-TTN | Mammalian cells | 118 |
pLEICS-121 | Amp | mCherry | Cpf1 | Staggered Cut | 5'-TTN | Mammalian cells | 118 |
pLEICS-122 | Amp | Puromycin | High-fidelityCas9 | All-in-one, Cut | 3'-NGG | Mammalian cells | 95 |
pLEICS-123 | Amp | Neomycin | High-fidelityCas9 | All-in-one, Cut | 3'-NGG | Mammalian cells | 95 |
pLEICS-124 | Amp | mEGFP | High-fidelityCas9 | All-in-one, Cut | 3'-NGG | Mammalian cells | 95 |
pLEICS-125 | Amp | mCherry | High-fidelityCas9 | All-in-one, Cut | 3'-NGG | Mammalian cells | 95 |
pLEICS-126 | Amp | Puromycin | High-fidelityCas9 | All-in-one, Cut | 3'-NGG | Mammalian cells | 95 |
pLEICS-127 | Amp | Neomycin | High-fidelityCas9 | All-in-one, Cut | 3'-NGG | Mammalian cells | 95 |
pLEICS-128 | Amp | mEGFP | High-fidelityCas9 | All-in-one, Cut | 3'-NGG | Mammalian cells | 95 |
pLEICS-129 | Amp | mCherry | High-fidelityCas9 | All-in-one, Cut | 3'-NGG | Mammalian cells | 95 |
pLEICS-140 | Amp | mEGFP | Cas9 | Nick (D10A), direct mutations of C to T or G to A | 3'-NGG | Mammalian cells | 95 |
pLEICS-141 | Amp | mCherry | Cas9 | Nick (D10A), direct mutations of C to T or G to A | 3'-NGG | Mammalian cells | 95 |
pLEICS-142 | Amp | Puromycin | Cas9 | Nick (D10A), direct mutations of C to T or G to A | 3'-NGG | Mammalian cells | 95 |
pLEICS-143 | Amp | Neomycin | Cas9 | Nick (D10A), direct mutations of C to T or G to A | 3'-NGG | Mammalian cells | 95 |
pLEICS-144 | Kan |
mEGFP Hygromycin |
Cas9 | All-in-one, Cut | 3'-NGG | Plant | 144 |
pLEICS-145 | Kan | mCherry Hygromycin |
Cas9 | All-in-one, Cut | 3'-NGG | Plant | 144 |
pLEICS-146 | Kan | mEGFP Hygromycin |
Cas9 | All-in-one, Nick (D10A) | 3'-NGG | Plant | 144 |
pLEICS-147 | Kan | mCherry Hygromycin |
Cas9 | All-in-one, Nick (D10A) | 3'-NGG | Plant | 144 |
pLEICS-148 | Kan | mEGFP Hygromycin |
High-fidelityCas9 | All-in-one, Cut | 3'-NGG | Plant | 144 |
pLEICS-149 | Kan | mCherry Hygromycin |
High-fidelityCas9 | All-in-one, Cut | 3'-NGG | Plant | 144 |
pLEICS-150 | Kan | Hygromycin | High-fidelityCas9 | All-in-one, Cut | 3'-NGG | Plant | 144 |