Protein Expression Laboratory (PROTEX)

The Protein Expression Laboratory (PROTEX) has been established to provide a high throughput service for cloning and small-scale protein expression trials. The laboratory is designed to serve the needs of structural biology, enzymology and molecular & cell biology including genome editing.

The pLEICS vectors, developed by PROTEX, can be used to express proteins in E. coli, mammalian cells, baculovirus, Pichia Pastoris, Drosophila, Xenopus and cell-free systems, with a choice of tags including: His6, S, GST, Trx, MBP, FLAG, TAP, HA, Myc, EG/C/YFP, Cherry, Mistic, FlAsH, luciferase, GB1, Halo, hBirA, miniSOG and APEX2.

PROTEX offers these services:

  • Clone into pLEICS vector(s) – choice of 137 vectors, including 42 CRISPR-Cas9 vectors
  • Preparation of designed mutations in protein coding regions

The laboratory will also provide expert advice in CRISPR-Cas9 genome editing, protein expression and selection of suitable regions to express.

Contact details

Tel: 0116 252 7089

Email: slm75@leicester.ac.uk

Address
Sharon L. Munday
Henry Wellcome Building
University of Leicester
Lancaser Rd.
Leicester
LE1 9HN