Department of Molecular and Cell Biology

Available Vectors

Currently, 137 vectors are available for protein expression in E. coli, mammalian cells, baculovirus, Pichia Pastoris, Drosophila, Xenopus and cell-free systems, with a choice of tags including: His6, S, GST, Trx, MBP, FLAG, TAP, HA, Myc, EG/C/YFP, Cherry, Mistic, FlAsH, luciferase, GB1, Halo, hBirA, miniSOG and APEX2.

We also have 42 all-in-one guide RNA/Cas9 expression vectors for mammalian and plant genome editing, with a choice of selection markers including EGFP, mCherry, puromycin, neomycin, zeocin, or blasticidin.

Available systems:


E. coli

* E. coli DE3 hosts: MUST express T7 RNA polymerase
* You MUST contact Dr. Julie Morrissey at the Department of Genetics to sign an MTA agreement before using pLEICS-151

Vector name AR Affinity Tag Protease Cleavage Family
pLEICS-01 Amp N-HIS6 TEV Extra SM C
pLEICS-02 Amp N-GST TEV Extra SM C
pLEICS-03 Kan N-HIS6 TEV Extra SM C
pLEICS-04 Kan N-GST TEV Extra SM C
pLEICS-05 Amp C-HIS6 TEV Extra ENLYFQ B
pLEICS-06 Amp N-HIS6/C-HIS6/S-Tag TEV Extra ENLYFQ B/C
pLEICS-07 Kan N-HIS6/S-Tag TEV Extra GA A
pLEICS-08 Kan N-GST/N-HIS6/S-Tag TEV Extra GA A
pLEICS-09 Amp N-Trx/N-HIS6/S-Tag TEV Extra GA A
pLEICS-10 Amp N-MBP TEV Extra GA A
pLEICS-14 Amp N-GST TEV Extra GA A
pLEICS-17 Kan N-GST-His6 TEV Extra GA A
pLEICS-18 Kan N-MBP TEV Extra GA A
pLEICS-43 Amp N-HIS6-MISTIC TEV Extra SM C
pLEICS-44 Kan N-HIS6-MISTIC TEV Extra SM C
pLEICS-45 Amp N-HIS6/mEGFP TEV Extra GA J/K
PLEICS-46 Kan N-GB1/HIS6 TEV Extra GA A
pLEICS-50 Amp C-GST TEV Extra ENLYFQ B
pLEICS-51 Kan N-HIS6-N-FlAsH TEV Extra SM C
pLEICS-52 Kan N-HIS6-N-FlAsH/mEGFP128 TEV Extra SM C
pLEICS-53 Kan N-HIS6-N-FlAsH/mEGFP129 TEV Extra SM C
pLEICS-54 Kan N-HIS6/N-mEGFP128 TEV Extra SM C
pLEICS-55 Kan N-HIS6/N-mEGFP129 TEV Extra SM C
pLEICS-56 Kan Super-mEGFP1-215 TEV Extra SM C
pLEICS-57 Kan N-HIS6/Super-mEGFP216-232 TEV Extra SM C
pLEICS-58 Amp mEGFP128 TEV Extra SM C
pLEICS-59 Amp mEGFP129 TEV Extra SM C
pLEICS-60 Kan N-HIS6-FlAsH Super-mEGFP1-215 TEV Extra SM C
pLEICS-61 Amp N-HIS6-FlAsH TEV Extra SM C
pLEICS-63 Amp C-Super folded mEGFP-HIS6 TEV Extra ENLYFQ B
pLEICS-64 Amp C-mEGFP-HIS6 TEV Extra ENLYFQ B
pLEICS-65 Amp C-Super folded mEGFP1-10/HIS6 TEV Extra ENLYFQ B
pLEICS-66 Amp C-Super folded mEGFP11/HIS6 TEV Extra ENLYFQ B
pLEICS-67 Amp N-HIS6/mCherry TEV Extra GA J/K
pLEICS-68 Amp N-HIS6/mEYFP TEV Extra GA J/K
pLEICS-69 Amp N-GST PreScission Protease Extra GP 69
pLEICS-89 Amp N-His6/S/TEV-Halo TEV Extra GA 89
pLEICS-90 Amp C-Halo-TEV-HIS6 TEV Extra ENLYFQ 90
pLEICS-91 Amp N-GB1/HIS6 TEV Extra GA A
pLEICS-92 Amp N-GST-His6 TEV Extra GA A
pLEICS-93 Amp N-HIS6/S-Tag TEV Extra GA A
pLEICS-151 Tet Vector for complementation in Pseudomonas aeuriginosa 151

Pichia Pastoris: GS115/KM71

Vector name AR Affinity Tag Protease Cleavage Family
pLEICS-31 Amp C-HIS6 TEV Extra ENLYFQ F
pLEICS-32 Amp C-HIS6 TEV Extra ENLYFQ G
pLEICS-33 Amp C-HIS6 TEV Extra ENLYFQ H
pLEICS-34 Amp C-HIS6 TEV Extra ENLYFQ I


Baculovirus

Vector name AR Affinity Tag Protease Cleavage Family
pLEICS-11 Amp N-HIS6 TEV Extra GA A
pLEICS-16 Amp N-GST TEV Extra GA A
pLEICS-47 Amp N-HIS10/3xFLAG TEV Extra GA A

Cell-free Wheat Germ Lysate

Vector name AR Affinity Tag Protease Cleavage Family
pLEICS-41 Amp N-HIS6 TEV Extra S C
pLEICS-42 Amp C-HIS6 TEV Extra ENLYFQ B

Mammalian Cells

Vector name AR Affinity Tag Protease Cleavage Family
pLEICS-12 Amp/Neo N-HIS10/3 x Flag TEV Extra GA A
pLEICS-13 Amp/Zeo N-HIS10/3 x Flag TEV Extra GA A
pLEICS-15 Kan/Neo N-TAP TEV Extra GA A
pLEICS-19 Amp N-HA TEV Extra GA A
pLEICS-20 Amp N-myc TEV Extra GA A
pLEICS-21 Kan/Neo N-EGFP TEV Extra GA D/E
pLEICS-22 Kan/neo N-EYFP TEV Extra GA D/E
pLEICS-23 Kan/Neo N-mCherry TEV Extra GA D/E
pLEICS-24 Kan/Neo N-ECFP TEV Extra GA D/E
pLEICS-25 Kan/neo N-mEGFP (A206K) TEV Extra GA D/E
pLEICS-26 Kan/Neo N-mEYFP (A206K) TEV Extra GA D/E
pLEICS-27 Kan/Neo N-mECFP (A206K) TEV Extra GA D/E
pLEICS-29 Kan/Neo C-EGFP TEV, Extra ENLYFQ 29T/29
pLEICS-30 Kan/Neo C-mCherry TEV, Extra ENLYFQ 29T/29
pLEICS-39 Amp/Neo N-His103 x Flag, C-Halo TEV, Extra GA at N-terminal 39
pLEICS-40 Amp/Neo N-His103 x Flag, C-hBirA TEV, Extra GA at N-terminal 40
pLEICS-49 Amp/Neo C-HIS4/3 x Flag TEV, Extra ENLYFQ 49
pLEICS-80 Amp/Puro copGFP/HA 80
pLEICS-85 Amp Luciferase 85
pLEICS-86 Amp Luciferase/enhancer 85
pLEICS-87 Amp Luciferase/Promoter 85
pLEICS-131 Amp/Neo N-His103 x Flag, C-EGFP-APEX2 131A/B/C/D
pLEICS-132 Amp/Neo N-His103 x Flag, C-mCherry-APEX2 131A/B/C/D
pLEICS-133 Amp/Neo N-His103 x Flag, C-EGFP-miniSOG 131A/B/C/D
pLEICS-134 Amp/Neo N-His103 x Flag, C-mCherry-miniSOG 131A/B/C/D
pLEICS-135 Amp/Puro PiyggBac Vector 135
pLEICS-136 Amp/Neo N-His103 x Flag, C-mEGFP TEV, Extra GA at N-terminal 136
pLEICS-137 Amp/Neo N-His103 x Flag, C-mCherry TEV, Extra GA at N-terminal 136
pLEICS-138 Amp/Neo N-mEGFP (A206K) TEV Extra GA D/E
pLEICS-139 Amp/Neo N-mCherry TEV Extra GA D/E

Stem Cell

Vector Name AR Tag Protease Cleavage Family
pLEICS-37 AMP 37
pLEICS-38 AMP 37
pLEICS-72 AMP EGFP 72
pLEICS-73 AMP EGFP 73
pLEICS-77 AMP 77
pLEICS-78 AMP 77
pLEICS-88 AMP mCherry, EGFP 73

Drosophila

* You MUST contact Dr. Flaviano Giorgini at Department of Genetics to sign MTA agreement first for using pLEICS-74, 75, 76 and 79

Vector Name AR Affinity Tag Protease Cleavage Family
pLEICS-74 Amp 74
pLEICS-75 AMP 74
pLEICS-76 AMP 76
pLEICS-79 AMP 79

Two-hybrid Vectors

Vector name AR Tag Host Family
pLEICS-35 Kan GAL4-BD S. Cerevisiae 35
pLEICS-36 Amp GAL4-AD S. Cerevisiae 36
pLEICS-81 Amp LexA S. Cerevisiae 81
pLEICS-82 Amp VP16 S. Cerevisiae 82
pLEICS-83 Amp VP16 Mammalian cells A
pLEICS-84 Amp GAL4 Mammalian cells A

CRISPR vectors

Vector Name AR Selection CRISPR Effector gRNA and Cleavage PAM Sequence Host Family
pLEICS-95 Amp EGFP Cas9 All-in-one, Nick (D10A) 3'-NGG Mammalian cells 95
pLEICS-96 Amp Puromycin Cas9 All-in-one, Nick (D10A) 3'-NGG Mammalian cells 95
pLEICS-97 Amp Puromycin Cas9 All-in-one, Cut 3'-NGG Mammalian cells 95
pLEICS-98 Kan Cas9 gRNA Zebrafish 98
pLEICS-99 Amp mCherry Cas9 All-in-one, Nick (D10A) 3'-NGG Mammalian cells 95
pLEICS-100 Amp mCherry Cas9 All-in-one, Cut 3'-NGG Mammalian cells 95
pLEICS-101 Amp mEGFP Cas9 All-in-one, Cut 3'-NGG Mammalian cells 95
pLEICS-102 Amp Neomycin Cas9 All-in-one, Nick (D10A) 3'-NGG Mammalian cells 95
pLEICS-103 Amp Neomycin Cas9 All-in-one, Cut 3'-NGG Mammalian cells 95
pLEICS-104 Amp Zeocin Cas9 All-in-one, Nick (D10A) 3'-NGG Mammalian cells 95
pLEICS-105 Amp Zeocin Cas9 All-in-one, Cut 3'-NGG Mammalian cells 95
pLEICS-106 Amp Puromycin Cas9 All-in-one, Cut 3'-NGG Lentivirus 95
pLEICS-107 Amp Puromycin Cas9 gRNA Lentivirus 95
pLEICS-108 Amp Blasticidin Cas9 Cas9 3'-NGG Lentivirus
pLEICS-109 Amp Puromycin Cas9 All-in-one, Inducible Cas9, Cut 3'-NGG Lentivirus 95
pLEICS-110 Amp Puromycin Cas9 All-in-one, Inducible Cas9, Cut 3'-NGG Mammalian cells 95
pLEICS-111 Amp Neomycin Cas9 All-in-one, Inducible Cas9, Cut 3'-NGG Mammalian cells 95
pLEICS-112 Amp Puromycin Cas9 All-in-one, Nick (D10A) 3'-NGAG Mammalian cells 95
pLEICS-113 Amp Puromycin Cas9 All-in-one, Cut 3'-NGAG Mammalian cells 95
pLEICS-114 Amp mCherry Cas9 All-in-one, Nick (D10A) 3'-NGAG Mammalian cells 95
pLEICS-115 Amp mCherry Cas9 All-in-one, Cut 3'-NGAG Mammalian cells 95
pLEICS-116 Amp Neomycin Cas9 All-in-one, Nick (D10A) 3'-NGAG Mammalian cells 95
pLEICS-117 Amp Neomycin Cas9 All-in-one, Cut 3'-NGAG Mammalian cells 95
pLEICS-118 Amp Puromycin Cpf1 Staggered Cut 5'-TTN Mammalian cells 118
pLEICS-119 Amp Neomycin Cpf1 Staggered Cut 5'-TTN Mammalian cells 118
pLEICS-120 Amp mEGFP Cpf1 Staggered Cut 5'-TTN Mammalian cells 118
pLEICS-121 Amp mCherry Cpf1 Staggered Cut 5'-TTN Mammalian cells 118
pLEICS-122 Amp Puromycin High-fidelityCas9 All-in-one, Cut 3'-NGG Mammalian cells 95
pLEICS-123 Amp Neomycin High-fidelityCas9 All-in-one, Cut 3'-NGG Mammalian cells 95
pLEICS-124 Amp mEGFP High-fidelityCas9 All-in-one, Cut 3'-NGG Mammalian cells 95
pLEICS-125 Amp mCherry High-fidelityCas9 All-in-one, Cut 3'-NGG Mammalian cells 95
pLEICS-126 Amp Puromycin High-fidelityCas9 All-in-one, Cut 3'-NGG Mammalian cells 95
pLEICS-127 Amp Neomycin High-fidelityCas9 All-in-one, Cut 3'-NGG Mammalian cells 95
pLEICS-128 Amp mEGFP High-fidelityCas9 All-in-one, Cut 3'-NGG Mammalian cells 95
pLEICS-129 Amp mCherry High-fidelityCas9 All-in-one, Cut 3'-NGG Mammalian cells 95
pLEICS-140 Amp mEGFP Cas9 Nick (D10A), direct mutations of C to T or G to A 3'-NGG Mammalian cells 95
pLEICS-141 Amp mCherry Cas9 Nick (D10A), direct mutations of C to T or G to A 3'-NGG Mammalian cells 95
pLEICS-142 Amp Puromycin Cas9 Nick (D10A), direct mutations of C to T or G to A 3'-NGG Mammalian cells 95
pLEICS-143 Amp Neomycin Cas9 Nick (D10A), direct mutations of C to T or G to A 3'-NGG Mammalian cells 95
pLEICS-144 Kan

mEGFP

Hygromycin		 Cas9 All-in-one, Cut 3'-NGG Plant 144
pLEICS-145 Kan mCherry
Hygromycin		 Cas9 All-in-one, Cut 3'-NGG Plant 144
pLEICS-146 Kan mEGFP
Hygromycin		 Cas9 All-in-one, Nick (D10A) 3'-NGG Plant 144
pLEICS-147 Kan mCherry
Hygromycin		 Cas9 All-in-one, Nick (D10A) 3'-NGG Plant 144
pLEICS-148 Kan mEGFP
Hygromycin		 High-fidelityCas9 All-in-one, Cut 3'-NGG Plant 144
pLEICS-149 Kan mCherry
Hygromycin		 High-fidelityCas9 All-in-one, Cut 3'-NGG Plant 144
pLEICS-150 Kan Hygromycin High-fidelityCas9 All-in-one, Cut 3'-NGG Plant 144

Retroviruses

Vector name AR Tag Protease Cleavage Family
pLEICS-94 Amp C-EGFP 94

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