Leicester Institute of Structural and Chemical Biology

Publications

Publications which have made use of the Midlands Regional Cryo-EM Facility:

  • Lancey C, Tehseen M, Bakshi S, Percival M, Takahashi M, Sobhy MA, RaducanuVS, Blair K, Muskett FW, Ragan TJ, Crehuet R, Hamdan SM, De Biasio A. Cryo-EM structure of human Pol κ bound to DNA and mono-ubiquitylated PCNA. Nat Commun. 2021 Oct 19;12(1):6095.

  • Smith SM, Larocque G, Wood KM, Morris KL, Roseman AM, Sessions RB, Royle SJ, Smith CJ. Multi-modal adaptor-clathrin contacts drive coated vesicle assembly. EMBO J. 2021 Oct 1;40(19):e108795.

  • Chi G, Ebenhoch R, Man H, Tang H, Tremblay LE, Reggiano G, Qiu X, Bohstedt T, Liko I, Almeida FG, Garneau AP, Wang D, McKinley G, Moreau CP, Bountra KD,Abrusci P, Mukhopadhyay SMM, Fernandez-Cid A, Slimani S, Lavoie JL, Burgess-Brown NA, Tehan B, DiMaio F, Jazayeri A, Isenring P, Robinson CV, Dürr KL. Phospho-regulation, nucleotide binding and ion access control in potassium-chloride cotransporters. EMBO J. 2021 Jul 15;40(14) PMID: 34031912
  • Figley MD, Gu W. Nanson JD, Shi Y, Sasaki Y, Cunnea K, Malde AK, Jia X, Luo Z, Saikot FK, Mosaiab T, Masic V, Holt S, Hartley-Tassell L, McGuinness HY, Manik MK, Bosanac T, Landsberg MJ, Kerry PS, Mobli M, Hughes RO, Milbrandt J, Kobe B, DiAntonio A, Ve T. SARM1 is a metabolic sensor activated by an increased NMN/NAD+ ratio to trigger axon degeneration. Neuron, 2021 March 2.
  • Fox JL, Hughes MA, Meng X, Sarnowska NA, Powley IR, Jukes-Jones R, Dinsdale D, Ragan TJ, Fairall L, Schwabe JWR, Morone N, Cain K, MacFarlane M. Cryo-EM structural analysis of FADD:Caspase-8 complexes defines the catalytic dimer architecture for co-ordinated control of cell fate. Nat Commun. 2021 Feb 5;12(1):819. doi: 10.1038/s41467-020-20806-9. PubMed [citation] PMID: 33547302
  • Zimmermann F, Serna M, Ezquerra A, Fernandez-Leiro R, Llorca O, Luders J.Assembly of the asymmetric human γ-tubulin ring complex by RUVBL1-RUVBL2 AAA ATPase. Sci Adv. 2020 Dec 18;6(51).
  • Millard CJ, Fairall L, Ragan TJ, Savva CG, Schwabe JWR. The topology of chromatin-binding domains in the NuRD deacetylase complex. Nucleic Acids Res. 2020 Dec 16;48(22):12972-12982. doi: 10.1093/nar/gkaa1121. PMID: 33264408
  • López-Perrote A, Hug N, González-Corpas A, Rodríguez CF, Serna M, García- Martín C, Boskovic J, Fernandez-Leiro R, Caceres JF, Llorca O. Regulation of RUVBL1-RUVBL2 AAA-ATPases by the nonsense-mediated mRNA decay factor DHX34, as evidenced by Cryo-EM. Elife. 2020 Nov 18;9:e63042. d
  • Higashi TL, Eickhoff P, Sousa JS, Locke J, Nans A, Flynn HR, Snijders AP, Papageorgiou G, O'Reilly N, Chen ZA, O'Reilly FJ, Rappsilber J, Costa A, Uhlmann F. A Structure-Based Mechanism for DNA Entry into the Cohesin Ring. Mol Cell. 2020 Jul 30:S1097-2765(20)30503-7.
  • Turnbull RE, Fairall L, Saleh A, Kelsall E, Morris KL, Ragan TJ, Savva CG, Chandru A, Millard CJ1,2, Makarova OV, Smith CJ, Roseman AM, Fry AM, Cowley SM, Schwabe JWR. The MiDAC histone deacetylase complex is essential for embryonic development and has a unique multivalent structure. Nat Commun. 2020 Jun 26;11(1):3252. doi: 10.1038/s41467-020-17078-8. PMID: 32591534
  • Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A. Structure of the processive human Pol δ holoenzyme. Nat Commun. 2020 Feb 28;11(1):1109.  
  • Song Y, Dagil L, Fairall L, Robertson N, Wu M, Ragan TJ, Savva CG, Saleh A,Morone N, Kunze MBA, Jamieson AG, Cole PA, Hansen DF, Schwabe JWR. Mechanism ofCrosstalk between the LSD1 Demethylase and HDAC1 Deacetylase in the CoRESTComplex. Cell Rep. 2020 Feb 25;30(8):2699-2711.e8.
  • Flydal MI, Alcorlo-Pagés M, Johannessen FG, Martínez-Caballero S, Skjærven L,Fernandez-Leiro R, Martinez A, Hermoso JA. Structure of full-length human phenylalanine hydroxylase in complex with tetrahydrobiopterin. Proc Natl Acad Sci U S A. 2019 Jun 4;116(23):11229-11234
  • Míguez Amil S, Jiménez-Ortega E, Ramírez-Escudero M, Talens-Perales D,Marín-Navarro J, Polaina J, Sanz-Aparicio J, Fernandez-Leiro R. The cryo-EM Structure of Thermotoga maritima β-Galactosidase: Quaternary Structure GuidesProtein Engineering. ACS Chem Biol. 2020 Jan 17;15(1):179-188. 
  • Savva CG, Clark AR, Naylor CE, Popoff MR, Moss DS, Basak AK, Titball RW,Bokori-Brown M. The pore structure of Clostridium perfringens epsilon toxin. Nat Commun. 2019 Jun 14;10(1):2641. doi: 10.1038/s41467-019-10645-8.
  • Falcon B, Zivanov J, Zhang W, Murzin AG, Garringer HJ, Vidal R, Crowther RA, Newell KL, Ghetti B, Goedert M, Scheres SHW. "Novel tau filament fold in chronic traumatic encephalopathy encloses hydrophobic molecules." Nature. 2019 Apr; 568(7752):420-423. doi: 10.1038/s41586-019-1026-5. Epub 2019 Mar 20. PubMed PMID: 30894745; PubMed Central PMCID: PMC6472968.
  • Savva CG, Clark AR, Naylor CE, Popoff MR, Moss DS, Basak AK, Titball RW, Bokori-Brown M. "The pore structure of Clostridium perfringens epsilon toxin." Nat Commun. 2019 Jun 14;10(1):2641. doi: 10.1038/s41467-019-10645-8. PubMed PMID: 31201325; PubMed Central PMCID: PMC6572795.
  • Míguez Amil S, Jiménez-Ortega E, Ramírez-Escudero M, Talens-Perales D, Marín-Navarro J, Polaina J, Sanz-Aparicio J, Fernandez-Leiro R. "The cryo-EM Structure of Thermotoga maritima β-Galactosidase: Quaternary Structure Guides Protein Engineering." ACS Chem Biol. 2020 Jan 17;15(1):179-188. doi: 10.1021/acschembio.9b00752. Epub 2019 Dec 24. PubMed PMID: 31874027.
  • Flydal MI, Alcorlo-Pagés M, Johannessen FG, Martínez-Caballero S, Skjærven L, Fernandez-Leiro R, Martinez A, Hermoso JA. "Structure of full-length human phenylalanine hydroxylase in complex with tetrahydrobiopterin." Proc Natl Acad Sci U S A. 2019 Jun 4;116(23):11229-11234. doi: 10.1073/pnas.1902639116. Epub 2019 May 22. PubMed PMID: 31118288; PubMed Central PMCID: PMC6561269.
  • Song Y, Dagil L, Fairall L, Robertson N, Wu M, Ragan TJ, Savva CG, Saleh A, Morone N, Kunze MBA, Jamieson AG, Cole PA, Hansen DF, Schwabe JWR. "Mechanism of Crosstalk between the LSD1 Demethylase and HDAC1 Deacetylase in the CoREST Complex." Cell Rep. 2020 Feb 25;30(8):2699-2711.e8. doi: 10.1016/j.celrep.2020.01.091. PubMed PMID: 32101746; PubMed Central PMCID: PMC7043024.
  • Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A. "Structure of the processive human Pol δ holoenzyme." Nat Commun. 2020 Feb 28;11(1):1109. doi:10.1038/s41467-020-14898-6. PubMed PMID: 32111820; PubMed Central PMCID: PMC7048817.
  • Turnbull RE, Fairall L, Saleh A, Kelsall E, Morris KL, Ragan TJ, Savva CG, Chandru A, Millard CJ1,2, Makarova OV, Smith CJ, Roseman AM, Fry AM, Cowley SM, Schwabe JWR. "The MiDAC histone deacetylase complex is essential for embryonic development and has a unique multivalent structure." Nat Commun. 2020 Jun 26;11(1):3252. doi: 10.1038/s41467-020-17078-8. PMID: 32591534.
  • Higashi TL, Eickhoff P, Sousa JS, Locke J, Nans A, Flynn HR, Snijders AP, Papageorgiou G, O'Reilly N, Chen ZA, O'Reilly FJ, Rappsilber J, Costa A, Uhlmann F. "A Structure-Based Mechanism for DNA Entry into the Cohesin Ring." Mol Cell. 2020 Jul 30:S1097-2765(20)30503-7.

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