Electron microscopy

cryo electron microscopy equipment in useThe Institute hosts The Midlands Regional Cryo-Electron Microscopy (cryo-EM) Facility, that sits at the centre of a Midlands Ecosystem with feeder instruments located in the Universities of Warwick, Birmingham, Nottingham and locally in Leicester (University and MRC Toxicology Unit). This state-of-the-art facility is located in the Henry Wellcome Building and is centred around the very latest 300 kV Titan Krios Cryo-EM with direct electron detecting camera systems. The facility also provides laboratory space for sample preparation including a dedicated temperature and humidity controlled lab which houses a Vitrobot Mark IV specimen preparation unit, allowing for the preparation of frozen grids under optimum conditions to achieve the best possible results. 

The facility also provides the necessary high-performance computing infrastructure necessary for analysing the very large volumes of data generated.

Facility includes:

  • FEI Titan Krios G3 300 kV transmission electron microscope
  • Falcon 3EC direct electron detection camera
  • GATAN K2 (planned upgrade to K3 in early 2019) BioQuantum direct electron detection filter camera
  • Vitrobot Mark IV – dual-sided specimen preparation unit
  • Custom built single-sided specimen preparation unit
  • Computational infrastructure - high performance dedicated cluster centred around 800 TB high speed storage paired with 32 GTX 1080Ti GPUs

See more about the history and development of the microscope.