Introduction to Molecular Techniques

Module code: MB7008

This module is designed to develop your experimental research skills and give you a comprehensive understanding of the theory and practice of key molecular biology techniques for working in bioscience laboratories. These techniques include:

• Molecular Cloning
• PCR and agarose gel electrophoresis
• Quantitative (q)PCR
• CRISPR_Cas9 gene editing
• Western Blotting 

A series of 5 laboratory practicals (accompanied by a parallel lecture course) will allow you to determine human genotypes, induce mutations, test gene expression and compare protein expression levels. In addition, this module will demonstrate how to use online bioinformatics/nucleic acid sequence databases for sequence comparisons and PCR primer design.