Core Biotechnology Services

Olympus IX81 - Inscoper

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This microscope is build around an inverted IX81 motorized microscope running Inscoper acquisition software. It is equipped with an Hamamatsu ORCA Flash 4.0 LT+ camera (pixel size 6.5x6.5 µm and 82 % peak quantum efficiency), DIC objectives, hardware ZDC (Z-Drift Compensation) and software autofocus, linear encoders for high xy-precision, CO2 and a stage incubator for live cell imaging.

The Inscoper software allows for imaging of fixed samples or live cells and includes a high-content screening option. This makes it possible to screen both large and small numbers of samples. It has fully automated image acquisition which allows the user to select the number of images per well, the number of wells and the different filter sets.

Data acquired on this system during high content screening can be converted for compatibility with ScanR analysis software for automated object detection and cell separation using histograms and gating settings to include or exclude data sets.

This system can handle slides, 35 mm dishes and multi well plates. A slide holder for 4 slides is available.

This system was originally funded by the Wolfson Foundation and installed in autumn 2007 as a combined ScanR/CellR system and mainly used for high content screening station. In 2022 it was upgraded with BBSRC funding as part of the Midlands Open Bioimaging (MOB) initiative. The upgrade included a new LED light source, new excitation and emission filters, a new camera and a new PC. The system was also upgraded with new acquisition software from Inscoper.

Current software: Inscoper 7.1.15

System specifications

CoolLED light source

  • 365 nm
  • 435 nm
  • 470 nm
  • 500 nm
  • 550 nm
  • 580 nm
  • 635 nm

Filter wheel with excitation filters

  • DAPI (376/30)
  • CFP (430/24)
  • GFP/FITC(480/30)
  • YFP (500/20)
  • mRFP/TRITC (555/25)
  • TxRed (560/40)
  • Cy5 (640/30)

Emission filter cubes on the microscope

  • DAPI/FITC/TRITC/Cy5; 418 - 450 nm, 505 - 532 nm, 577 - 610 nm, 664.5 - 725 nm (Chroma 89402m)
  • CFP/YFP; 455 - 484 nm, 520 - 554 nm (Chroma 29017m)
  • GFP/RFP;  
  • HqFITC; 510 - 565 nm
  • Cy5 (Dichroic 660LP, emission 700/75m)

Olympus objectives

There are a list of objectives available for the Olympus imaging systems.

See the Olympus objectives
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