People

Dr James Higgins

Associate Professor

School/Department: Genetics and Genome Biology, Department of

Telephone: +44 (0)116 2231296

Email: jh555@leicester.ac.uk

Address: Adrian Building, University Road, Leicester. LE1 7RH

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UKRI Grants

Profile

I received a BSc (Hons) in Biology from the University of Nottingham (1997) where I developed an interest in plant genetics. I pursued applying this knowledge to improve plant varieties by undertaking an MSc in Crop protection at the University of Bristol (1998). This was followed by a PhD at HRI-Warwick to understand and potentially delay the post-harvest yellowing of broccoli (2002). As a post-doctoral fellow I worked in the groups of Professor Chris Franklin and Dr. Gareth Jones (University of Birmingham) to identify and characterise key genes involved in meiotic recombination in the model plant Arabidopsis thaliana. The knowledge and tools were transferred to barley (Hordeum vulgare) to open up inaccessible areas of the genome to recombination so that desirable traits can be bred together and undesirable traits selected out. I became a lecturer at the University of Leicester in 2013 and was promoted to Associate Professor in 2017. I'm currently pursuing an interest in crop genetics as well as using model systems to gain a greater understanding of meiotic recombination to improve crop varieties for the challenges of the 21st Century.

Research

The focus of our research is to unravel the mechanisms governing meiotic crossover frequency and distribution in model plants and crop species including Arabidopsis, barley, wheat and rice. Genetic cytogenetic and genomic techniques are used to answer fundamental biological questions regarding the function of meiotic genes that ensure correct chromosome pairing synapsis and crossing over to promote balanced segregation of chromosomes into haploid gametes. One of the outstanding mysteries is how the synaptonemal complex proteins mediate crossover patterning and we aim to further elucidate and translate this knowledge into breeding programmes.

Publications

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SD Desjardins, J Simmonds, I Guterman, K Kanyuka, AJ Burridge, AJ Tock, E Sanchez-Moran, FCH Franklin, IR Henderson, KJ Edwards, C Uauy, JD Higgins. (2022) FANCM promotes class I interfering crossovers and suppresses class II non-interfering crossovers in wheat meiosis. Nature Communications: 13(1):3644 https://doi.org/10.1038/s41467-022-31438-6

AC Tidy, I Ferjentsikova, G Vizcay-Barrena, B Liu, W Yin, JD Higgins, X Jie, D Zhang, D Geelen, ZA Wilson. (2022) Journal of Experimental Botany.  https://doi.org/10.1093/jxb/erac225

AJ Tock, DM Holland, W Jiang, K Osman, E Sanchez-Moran, JD Higgins, KJ Edwards, C Uauy, FCH Franklin, IR Henderson. (2021). Crossover-active regions of the wheat genome are distinguished by DMC1, the chromosome axis, H3K27me3, and signatures of adaptation. Genome Research 31: 1614-1628. https://doi.org/10.1101/gr.273672.120

MG France, J Enderle, S Röhrig, H Puchta, FCH Franklin, JD Higgins. (2021). ZYP1 is required for obligate cross-over formation and cross-over interference in Arabidopsis. Proceedings of the National Academy of Sciences. 118. https://doi.org/10.1073/pnas.2021671118

K Osman, U Algopishi, JD Higgins, IR Henderson, KJ Edwards, FCH Franklin, E Sanchez-Moran. (2021). Distal bias of meiotic crossovers in hexaploid bread wheat reflects spatio-temporal asymmetry of the meiotic program. Front Plant Sci. 120. https://doi.org/10.3389/fpls.2021.631323

AR Blackwell, J Dluzewska, M Szymanska‐Lejman, S Desjardins, AJ Tock, N Kbiri, C Lambing, EJ Lawrence, T Bieluszewski, B Rowan, JD Higgins, PA Ziolkowski, IR Henderson. (2020). MSH2 shapes the meiotic crossover landscape in relation to interhomolog polymorphism in Arabidopsis. The EMBO journal 39: e104858. https://doi.org/10.15252/embj.2020104858

SD Desjardins, DE Ogle, MA Ayoub, S Heckmann, IR Henderson, KJ Edwards, JD Higgins JD. (2020). MutS homologue 4 and MutS homologue 5 maintain the obligate crossover in wheat despite stepwise gene loss following polyploidisation. Plant Physiology 183: 1545-1558. https://doi.org/10.1104/pp.20.00534

PJ Seear, MG France, CL Gregory, D Heavens, R Schmickl, L Yant, JD Higgins. (2020). A novel allele of ASY3 is associated with greater meiotic stability in autotetraploid Arabidopsis lyrata. Plos Genet 16: e1008900. https://doi.org/10.1371/journal.pgen.1008900

C Di Dio, V Longobardi, G Zullo, P Parma, A Pauciullo, A Perucatti, JD Higgins, A Iannuzzi. (2020). Analysis of meiotic segregation by triple-color fish on both total and motile sperm fractions in a t(1p;18) river buffalo bull. Plos One 15: e0232592.  https://doi.org/10.1371/journal.pone.0232592

C Lambing, AJ Tock, SD Topp, K Choi, PC Kuo, X Zhao, K Osman, JD Higgins, FCH Franklin, IR Henderson. (2020). Interacting genomic landscapes of REC8-cohesin, chromatin, and meiotic recombination in Arabidopsis. The Plant Cell 32: 1218-1239.   https://doi.org/10.1105/tpc.19.00866

R Fu, C Wang, H Shen, J Zhang, JD Higgins, W Liang. (2020). Rice OsBRCA2 is required for DNA double-strand break repair in meiotic cells. Front Plant Sci: 1800. https://doi.org/10.3389/fpls.2020.600820

S Desjardins, K Kanyuka, JD Higgins. (2020). A cytological analysis of wheat meiosis targeted by virus-induced gene silencing (VIGS). Plant Meiosis, pp. 319-330. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9818-0_22

L-J Gardiner, LU Wingen, P Bailey, R Joynson, T Brabbs, J Wright, JD Higgins, N Hall, S Griffiths, BJ Clavijo. (2019). Analysis of the recombination landscape of hexaploid bread wheat reveals genes controlling recombination and gene conversion frequency. Genome Biology 20: 1-16.    https://doi.org/10.1186/s13059-019-1675-6

S Marburger, P Monnahan, PJ Seear, SH Martin, J Koch, P Paajanen, M Bohutínská, JD Higgins, R Schmickl, L Yant. (2019). Interspecific introgression mediates adaptation to whole genome duplication. Nature communications 10: 1-11.  https://doi.org/10.1038/s41467-019-13159-5

J Zhang, C Wang, JD Higgins, Y-J Kim, S Moon, K-H Jung, S Qu, W Liang. (2019). A multiprotein complex regulates interference-sensitive crossover formation in rice. Plant physiology 181: 221-235. https://doi.org/10.1104/pp.19.00082

AS Shunmugam, V Bollina, S Dukowic-Schulze, PK Bhowmik, C Ambrose, JD Higgins, C Pozniak, AG Sharpe, K Rozwadowski, S Kagale. (2018). MeioCapture: an efficient method for staging and isolation of meiocytes in the prophase I sub-stages of meiosis in wheat. BMC Plant Biol 18: 1-12.  https://doi.org/10.1186/s12870-018-1514-z

J Walker, H Gao, J Zhang, B Aldridge, M Vickers, JD Higgins, X Feng. (2018). Sexual-lineage-specific DNA methylation regulates meiosis in Arabidopsis. Nat Genet 50: 130-137. https://doi.org/10.1038/s41588-017-0008-5

VI Simón-Porcar, JL Silva, S Meeus, JD Higgins, M Vallejo-Marín. (2017). Recent autopolyploidisation in a naturalised population of Mimulus guttatus (Phrymaceae). Bot J Linn Soc 185: 189-207. https://doi.org/10.1093/botlinnean/box052

C Wang, JD Higgins, Y He, P Lu, D Zhang, W Liang. (2017). Resolvase OsGEN1 mediates DNA repair by homologous recombination. Plant physiology 173: 1316-1329. https://doi.org/10.1104%2Fpp.16.01726

A Sepsi, JD Higgins, JS Heslop‐Harrison, T Schwarzacher. (2017). CENH3 morphogenesis reveals dynamic centromere associations during synaptonemal complex formation and the progression through male meiosis in hexaploid wheat. The Plant Journal 89: 235-249.  https://doi.org/10.1111/tpj.13379

M Fu, C Wang, F Xue, JD Higgins, M Chen, D Zhang, W Liang. (2016). The DNA topoisomerase VI–B subunit OsMTOPVIB is essential for meiotic recombination initiation in rice. Molecular Plant 9: 1539-1541. https://doi.org/10.1016/j.molp.2016.07.006

I Colas, M Macaulay, JD Higgins, D Phillips, A Barakate, M Posch, SJ Armstrong, FCH Franklin, C Halpin, R Waugh. (2016). A spontaneous mutation in MutL‐Homolog 3 (HvMLH3) affects synapsis and crossover resolution in the barley desynaptic mutant des10. New Phytologist 212: 693-707. https://doi.org/10.1111/nph.14061

Y He, C Wang, JD Higgins, J Yu, J Zong, P Lu, D Zhang, W Liang. (2016). MEIOTIC F-BOX is essential for male meiotic DNA double-strand break repair in rice. The Plant Cell 28: 1879-1893. https://doi.org/10.1105/tpc.16.00108

K Bomblies, JD Higgins, L Yant. (2015). Meiosis evolves: adaptation to external and internal environments. New Phytologist 208: 306-323.  https://doi.org/10.1111/nph.13499

C Lambing, K Osman, K Nuntasoontorn, A West, JD Higgins, GP Copenhaver, J Yang, SJ Armstrong, K Mechtler, E Roitinger. (2015). Arabidopsis PCH2 mediates meiotic chromosome remodelling and maturation of crossovers. Plos Genet 11: e1005372.  https://doi.org/10.1371/journal.pgen.1005372

JD Higgins, K Osman, GH Jones, FCH Franklin. (2014). Factors underlying restricted crossover localisation in barley meiosis. Annual Review of Genetics 48: 29-47. https://doi.org/10.1146/annurev-genet-120213-092509

A Barakate, JD Higgins, S Vivera, J Stephens, RM Perry, L Ramsay, I Colas, H Oakey, R Waugh, FCH Franklin. (2014). The synaptonemal complex protein ZYP1 is required for imposition of meiotic crossovers in barley. The Plant Cell 26: 729-740.   https://doi.org/10.1105/tpc.113.121269

JD Higgins, KM Wright, K Bomblies, C Franklin. (2014). Cytological techniques to analyse meiosis in Arabidopsis arenosa for investigating adaptation to polyploidy. Front Plant Sci 4: 546.  https://dx.doi.org/10.3389/fpls.2013.00546

L Yant, JD Hollister, KM Wright, BJ Arnold, JD Higgins, FCH Franklin, K Bomblies. (2013). Meiotic adaptation to genome duplication in Arabidopsis arenosa. Curr Biol 23: 2151-2156.  https://doi.org/10.1016/j.cub.2013.08.059

K Choi, X Zhao, KA Kelly, O Venn, JD Higgins, NE Yelina, TJ Hardcastle, PA Ziolkowski, GP Copenhaver, FCH Franklin. (2013). Arabidopsis meiotic crossover hot spots overlap with H2A.Z nucleosomes at gene promoters. Nat Genet 45: 1327-1336.  https://doi.org/10.1038/ng.2766

X Li, Y Chang, X Xin, C Zhu, X Li, JD Higgins, C Wu. (2013). Replication protein A2c coupled with replication protein A1c regulates crossover formation during meiosis in rice. The Plant Cell 25: 3885-3899.  https://doi.org/10.1105/tpc.113.118042

D Phillips, J Wnetrzak, C Nibau, A Barakate, L Ramsay, F Wright, JD Higgins, RM Perry, G Jenkins. (2013). Quantitative high resolution mapping of HvMLH3 foci in barley pachytene nuclei reveals a strong distal bias and weak interference. J Exp Bot 64: 2139-2154.  https://doi.org/10.1093/jxb/ert079

JD Higgins. (2013). Analysing meiosis in barley. Plant meiosis, pp. 135-144. Humana Press, Totowa, NJ.  https://doi.org/10.1007/978-1-62703-333-6_14

JD Higgins, RM Perry, A Barakate, L Ramsay, R Waugh, C Halpin, SJ Armstrong, FCH Franklin. (2012). Spatiotemporal asymmetry of the meiotic program underlies the predominantly distal distribution of meiotic crossovers in barley. The Plant Cell 24: 4096-4109.  https://doi.org/10.1105/tpc.112.102483

A Knoll, JD Higgins, K Seeliger, SJ Reha, NJ Dangel, M Bauknecht, S Schröpfer, FCH Franklin, H Puchta. (2012). The Fanconi anemia ortholog FANCM ensures ordered homologous recombination in both somatic and meiotic cells in Arabidopsis. The Plant Cell 24: 1448-1464. https://doi.org/10.1105/tpc.112.096644

M Ferdous, JD Higgins, K Osman, C Lambing, E Roitinger, K Mechtler, SJ Armstrong, R Perry, M Pradillo, N Cuñado. (2012). Inter-homolog crossing-over and synapsis in Arabidopsis meiosis are dependent on the chromosome axis protein AtASY3. Plos Genet 8: e1002507. https://doi.org/10.1371/journal.pgen.1002507

K Osman, JD Higgins, E Sanchez‐Moran, SJ Armstrong, FCH Franklin. (2011). Pathways to meiotic recombination in Arabidopsis thaliana. New Phytologist 190: 523-544.  https://doi.org/10.1111/j.1469-8137.2011.03665.x

Z Chen, JD Higgins, JTL Hui, J Li, FCH Franklin, F Berger. (2011). Retinoblastoma protein is essential for early meiotic events in Arabidopsis. The EMBO journal 30: 744-755. https://doi.org/10.1038/emboj.2010.344

JD Higgins, M Ferdous, K Osman, FCH Franklin. (2011). The RecQ helicase AtRECQ4A is required to remove inter‐chromosomal telomeric connections that arise during meiotic recombination in Arabidopsis. The Plant Journal 65: 492-502. https://doi.org/10.1111/j.1365-313x.2010.04438.x

JD Higgins, J Vignard, R Mercier, AG Pugh, FCH Franklin, GH Jones. (2008). AtMSH5 partners AtMSH4 in the class I meiotic crossover pathway in Arabidopsis thaliana, but is not required for synapsis. The Plant Journal 55: 28-39. https://doi.org/10.1111/j.1365-313x.2008.03470.x

JD Higgins, EF Buckling, FCH Franklin, GH Jones. (2008). Expression and functional analysis of AtMUS81 in Arabidopsis meiosis reveals a role in the second pathway of crossing‐over. The Plant Journal 54: 152-162. https://doi.org/10.1111/j.1365-313x.2008.03403.x

E Sanchez-Moran, K Osman, JD Higgins, M Pradillo, N Cunado, G Jones, F Franklin. (2008). ASY1 coordinates early events in the plant meiotic recombination pathway. Cytogenet Genome Res 120: 302-312. https://doi.org/10.1159/000121079

F Franklin, JD Higgins, E Sanchez-Moran, S Armstrong, K Osman, N Jackson, G Jones. (2006). Control of meiotic recombination in Arabidopsis: role of the MutL and MutS homologues. Biochemical Society Transactions 34 542-544.  https://doi.org/10.1042/bst0340542

K Osman, E Sanchez-Moran, JD Higgins, GH Jones, FCH Franklin. (2006). Chromosome synapsis in Arabidopsis: analysis of the transverse filament protein ZYP1 reveals novel functions for the synaptonemal complex. Chromosoma 115: 212-219. https://doi.org/10.1007/s00412-005-0042-4

JD Higgins, H Newbury, D Barbara, S Muthumeenakshi, I Puddephat. (2006). The production of marker-free genetically engineered broccoli with sense and antisense ACC synthase 1 and ACC oxidases 1 and 2 to extend shelf-life. Mol Breeding 17: 7-20. https://doi.org/10.1007/s11032-005-0237-7

JD Higgins, E Sanchez-Moran, SJ Armstrong, GH Jones, FCH Franklin. (2005). The Arabidopsis synaptonemal complex protein ZYP1 is required for chromosome synapsis and normal fidelity of crossing over. Genes & Dev 19: 2488-2500. https://doi.org/10.1101%2Fgad.354705

E Sánchez-Morán, R Mercier, JD Higgins, S Armstrong, G Jones, F Franklin. (2005). A strategy to investigate the plant meiotic proteome. Cytogenet Genome Res 109: 181-189. https://doi.org/10.1159/000082398

JD Higgins, SJ Armstrong, FCH Franklin, GH Jones. (2004). The Arabidopsis MutS homolog AtMSH4 functions at an early step in recombination: evidence for two classes of recombination in Arabidopsis. Genes & Dev 18: 2557-2570. https://doi.org/10.1101%2Fgad.317504

PJ Jewess, JD Higgins, KJ Berry, SR Moss, AB Boogaard, BPS Khambay. (2002). Herbicidal action of 2‐hydroxy‐3‐alkyl‐1, 4‐naphthoquinones. Pest Management Science (formerly Pesticide Science) 58: 234-242. https://doi.org/10.1002/ps.428

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Supervision

We are interested in investigating factors controlling meiotic recombination in plants including Arabidopsis, barley and wheat. At biological level this is important for plant fertility, especially with a warming climate, but also the fundamental mechanisms governing crossover number and position.

PhD projects are offered in these general areas; applicants are encouraged to think of projects that would match their own areas of interest and complement our other activities. Please contact me for further information or to discuss project ideas. Active PhD projects are advertised on findaphd.com

Teaching

I convene and teach the MSc in Molecular Genetics. I also teach 2nd year Bioinformatics and 1st year Biodiversity and behaviour

Press and media

Meiosis and recombination in plants

Activities

I am a member of the Genetics Society and Royal Society of Biology

Conferences

I recently organised the British Meiosis Meeting conference at the University of Leicester (May 2022) and have attended the EMBO Meiosis conference since 2005, the Plant and Animal Genome Conference since 2009 and Gordon Meiosis Conference since 2006. 

Media coverage

Scientists discover a new flower of Shetland

Qualifications

PhD University of Birmingham (2002)

MSc University of Bristol (1998)

BSc University of Nottingham (1997)

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