People
Dr David Guttery
Lecturer in Cancer Early Detection
School/Department: Genetics and Genome Biology, Department of
Telephone: +44 (0)116 252 3181
Email: dsg6@leicester.ac.uk
Address: Leicester Cancer Research Centre, College of Life Sciences, University of Leicester, Room 425, Level 4, Robert Kilpatrick Clinical Sciences Building, Leicester Royal Infirmary, PO BOX 65, Leicester LE2 7LX, United Kingdom
Profile
Dr Guttery is the lecturer in Cancer Early Detection in the Leicester Cancer Research Centre (LCRC), whose research focuses on translational studies using artificial intelligence and liquid biopsy for the detection and monitoring of cancer.
Dr Guttery is also the postgraduate tutor for the LCRC and responsible for recruitment, development and well-being of all PhD students who are trained in the department.
Dr Guttery’s research also focuses on the cell biology of the malaria parasite Plasmodium, particularly during the mosquito stages of its life-cycle and how reversible protein phosphorylation plays a key role during these stages.
Research
Dr Guttery’s research is focused on the utility of circulating tumour DNA, combined with artificial intelligence (AI), towards earlier detection and monitoring of cancer.
Over the past ten years, Dr Guttery has worked on earlier detection of acquired resistance to hormone therapies in metastatic breast cancer, as part of Professor Jacqui Shaw's CRUK-funded programme.
This has now expanded into using ctDNA for monitoring endometrial cancer relapse, as part of a wider gynaecological research group led by Dr Esther Moss.
Dr Guttery has recently developed a collaboration with Dr Harriet Walter and Dr Oladejo Olaleye, looking at improving PET/CT accuracy post-treatment, and predicting relapse through the combination of ctDNA analysis with AI. Dr Guttery also has an exciting collaboration ongoing with Professor Yudong Zhang of the School of Informatics that looks to improve medical imaging methods for breast and other cancers using machine-learning.
Dr Guttery is also focused on the development of the malaria parasite, Plasmodium in the mosquito midgut, unravelling the cell biology and molecular mechanisms regulating cell division and reversible protein phosphorylation, with a view to developing new therapeutics to combat the disease, in collaboration with Professor Rita Tewari of the University of Nottingham.
Publications
1. Yu, X. and Ren, Z. and Guttery, D.S. and Zhang, Y.D. (2024) DF-dRVFL: A novel deep feature based classifier for breast mass classification. Multimed Tools Appl 83, 14393-14422. 10.1007/s11042-023-15864-2
2. Shaw, J.A., Page, K., Wren, E., de Bruin, E.C., Kalashnikova, E., Hastings, R. et al. (2024) Serial Postoperative Circulating Tumor DNA Assessment Has Strong Prognostic Value During Long-Term Follow-Up in Patients With Breast Cancer. JCO Precis Oncol 8, e2300456. 10.1200/PO.23.00456
3. Guttery, D.S. and Zeeshan, M. and Holder, A.A. and Tewari, R. (2024) The molecular mechanisms driving Plasmodium cell division. Biochem Soc Trans 52, 593-602. 10.1042/BST20230403
4. Darif, N.D., Ganter, M., Dziekan, J.M., Kilian, N., Brancucci, N., Ng, C. et al. (2024) BioMalPar XX: looking back on, and forward from, 20 years of malaria research. Trends Parasitol. 10.1016/j.pt.2024.06.012
5. Zeeshan, M., Rea, E., Abel, S., Vukusic, K., Markus, R., Brady, D. et al. (2023) Plasmodium ARK2 and EB1 drive unconventional spindle dynamics, during chromosome segregation in sexual transmission stages. Nat Commun 14, 5652. 10.1038/s41467-023-41395-3
6. Stebbing, J., Takis, P.G., Sands, C.J., Maslen, L., Lewis, M.R., Gleason, K. et al. (2023) Comparison of phenomics and cfDNA in a large breast screening population: the Breast Screening and Monitoring Study (BSMS). Oncogene 42, 825-832. 10.1038/s41388-023-02591-z
7. Guttery, D.S. and Zeeshan, M. and Holder, A.A. and Tromer, E.C. and Tewari, R. (2023) Meiosis in Plasmodium: how does it work? Trends Parasitol 39, 812-821. 10.1016/j.pt.2023.07.002
8. Yu, X. and Wang, S.H. and Gorriz, J.M. and Jiang, X.W. and Guttery, D.S. and Zhang, Y.D. (2022) PeMNet for Pectoral Muscle Segmentation. Biology (Basel) 11. 10.3390/biology11010134
9. Trethewey, C.S., Walter, H.S., Alqahtani, A.N.M., Schmid, R., Guttery, D.S., Griffin, Y. et al. (2022) Limitations of Monitoring Disease Progression Using Circulating Tumor DNA in Lymphoma: An Example From Primary Cutaneous DLBCL Leg-type. Hemasphere 6, e690. 10.1097/HS9.0000000000000690
10. Harrod, A., Lai, C.F., Goldsbrough, I., Simmons, G.M., Oppermans, N., Santos, D.B. et al. (2022) Genome engineering for estrogen receptor mutations reveals differential responses to anti-estrogens and new prognostic gene signatures for breast cancer. Oncogene 41, 4905-4915. 10.1038/s41388-022-02483-8
11. Guttery, D.S. and Zeeshan, M. and Ferguson, D.J.P. and Holder, A.A. and Tewari, R. (2022) Division and Transmission: Malaria Parasite Development in the Mosquito. Annu Rev Microbiol 76, 113-134. 10.1146/annurev-micro-041320-010046
12. Fernandez-Garcia, D., Nteliopoulos, G., Hastings, R.K., Rushton, A., Page, K., Allsopp, R.C. et al. (2022) Shallow WGS of individual CTCs identifies actionable targets for informing treatment decisions in metastatic breast cancer. Br J Cancer 127, 1858-1864. 10.1038/s41416-022-01962-9
13. Zhang, Y.D. and Satapathy, S.C. and Wu, D. and Guttery, D.S. and Gorriz, J.M. and Wang, S.H. (2021) Improving ductal carcinoma in situ classification by convolutional neural network with exponential linear unit and rank-based weighted pooling. Complex Intell Systems 7, 1295-1310. 10.1007/s40747-020-00218-4
14. Zeeshan, M., Pandey, R., Subudhi, A.K., Ferguson, D.J.P., Kaur, G., Rashpa, R. et al. (2021) Protein phosphatase 1 regulates atypical mitotic and meiotic division in Plasmodium sexual stages. Commun Biol 4, 760. 10.1038/s42003-021-02273-0
15. Yu, X. and Kang, C. and Guttery, D.S. and Kadry, S. and Chen, Y. and Zhang, Y.D. (2021) ResNet-SCDA-50 for Breast Abnormality Classification. IEEE/ACM Trans Comput Biol Bioinform 18, 94-102. 10.1109/TCBB.2020.2986544
16. Xia, S.J. and Gao, B.Z. and Wang, S.H. and Guttery, D.S. and Li, C.D. and Zhang, Y.D. (2021) Modeling of diagnosis for metabolic syndrome by integrating symptoms into physiochemical indexes. Biomed Pharmacother 137, 111367. 10.1016/j.biopha.2021.111367
17. Wang, S.H. and Nayak, D.R. and Guttery, D.S. and Zhang, X. and Zhang, Y.D. (2021) COVID-19 classification by CCSHNet with deep fusion using transfer learning and discriminant correlation analysis. Inf Fusion 68, 131-148. 10.1016/j.inffus.2020.11.005
18. Relton, A. and Collins, A. and Guttery, D.S. and Gorsia, D.N. and McDermott, H.J. and Moss, E.L. (2021) Patient acceptability of circulating tumour DNA testing in endometrial cancer follow-up. Eur J Cancer Care (Engl) 30, e13429. 10.1111/ecc.13429
19. Page, K., Martinson, L.J., Hastings, R.K., Fernandez-Garcia, D., Gleason, K.L.T., Gray, M.C. et al. (2021) Prevalence of ctDNA in early screen-detected breast cancers using highly sensitive and specific dual molecular barcoded personalised mutation assays. Ann Oncol 32, 1057-1060. 10.1016/j.annonc.2021.04.018
20. Page, K., Martinson, L.J., Fernandez-Garcia, D., Hills, A., Gleason, K.L.T., Gray, M.C. et al. (2021) Circulating Tumor DNA Profiling From Breast Cancer Screening Through to Metastatic Disease. JCO Precis Oncol 5. 10.1200/PO.20.00522
21. Nteliopoulos, G., Page, K., Hills, A., Howarth, K., Emmett, W., Green, E. et al. (2021) Comparison of two targeted ultra-deep sequencing technologies for analysis of plasma circulating tumour DNA in endocrine-therapy-resistant breast cancer patients. Breast Cancer Res Treat 188, 465-476. 10.1007/s10549-021-06220-9
22. Liu, S., Zhao, L., Wang, X., Xin, Q., Zhao, J., Guttery, D.S. et al. (2021) Deep Spatio-Temporal Representation and Ensemble Classification for Attention Deficit/Hyperactivity Disorder. IEEE Trans Neural Syst Rehabil Eng 29, 1-10. 10.1109/TNSRE.2020.3019063
23. Kang, C., Yu, X., Wang, S.H., Guttery, D.S., Pandey, H.M., Tian, Y. et al. (2021) A Heuristic Neural Network Structure Relying on Fuzzy Logic for Images Scoring. IEEE Trans Fuzzy Syst 29, 34-45. 10.1109/TFUZZ.2020.2966163
24. Hastings, R.K., Openshaw, M.R., Vazquez, M., Moreno-Cardenas, A.B., Fernandez-Garcia, D., Martinson, L. et al. (2021) Longitudinal whole-exome sequencing of cell-free DNA for tracking the co-evolutionary tumor and immune evasion dynamics: longitudinal data from a single patient. Ann Oncol 32, 681-684. 10.1016/j.annonc.2021.02.007
25. Collins, A. and Taylor, A. and Guttery, D.S. and Moss, E.L. (2021) Innovative Follow-up Strategies for Endometrial Cancer. Clin Oncol (R Coll Radiol) 33, e383-e392. 10.1016/j.clon.2021.06.001
26. Zeeshan, M., Pandey, R., Ferguson, D.J.P., Tromer, E.C., Markus, R., Abel, S. et al. (2020) Real-time dynamics of Plasmodium NDC80 reveals unusual modes of chromosome segregation during parasite proliferation. J Cell Sci 134. 10.1242/jcs.245753
27. Polymeros, K., Guttery, D.S., Hew, R., Bishop, R., Stannard, E., Macip, S. et al. (2020) Differences in the molecular profile of endometrial cancers from British White and British South Asian women. PLoS One 15, e0233900. 10.1371/journal.pone.0233900
28. Openshaw, M.R., Suwaidan, A.A., Ottolini, B., Fernandez-Garcia, D., Richards, C.J., Page, K. et al. (2020) Longitudinal monitoring of circulating tumour DNA improves prognostication and relapse detection in gastroesophageal adenocarcinoma. Br J Cancer 123, 1271-1279. 10.1038/s41416-020-1002-8
29. Moss, E.L., Gorsia, D.N., Collins, A., Sandhu, P., Foreman, N., Gore, A. et al. (2020) Utility of Circulating Tumor DNA for Detection and Monitoring of Endometrial Cancer Recurrence and Progression. Cancers (Basel) 12. 10.3390/cancers12082231
30. Guttery, D.S., Ramaprasad, A., Ferguson, D.J.P., Zeeshan, M., Pandey, R., Brady, D. et al. (2020) MRE11 Is Crucial for Malaria Parasite Transmission and Its Absence Affects Expression of Interconnected Networks of Key Genes Essential for Life. Cells 9. 10.3390/cells9122590
31. Guttery, D.S., Pandey, R., Ferguson, D.J., Wall, R.J., Brady, D., Gupta, D. et al. (2020) Plasmodium DEH is ER-localized and crucial for oocyst mitotic division during malaria transmission. Life Sci Alliance 3. 10.26508/lsa.202000879
32. Wang, S.H., Xie, S., Chen, X., Guttery, D.S., Tang, C., Sun, J. et al. (2019) Alcoholism Identification Based on an AlexNet Transfer Learning Model. Front Psychiatry 10, 205. 10.3389/fpsyt.2019.00205
33. Roques, M., Stanway, R.R., Rea, E.I., Markus, R., Brady, D., Holder, A.A. et al. (2019) Plasmodium centrin PbCEN-4 localizes to the putative MTOC and is dispensable for malaria parasite proliferation. Biol Open 8. 10.1242/bio.036822
34. Page, K. and Shaw, J.A. and Guttery, D.S. (2019) The liquid biopsy: towards standardisation in preparation for prime time. Lancet Oncol 20, 758-760. 10.1016/S1470-2045(19)30310-9
35. Moore, D.A., Sereno, M., Das, M., Baena Acevedo, J.D., Sinnadurai, S., Smith, C. et al. (2019) In situ growth in early lung adenocarcinoma may represent precursor growth or invasive clone outgrowth-a clinically relevant distinction. Mod Pathol 32, 1095-1105. 10.1038/s41379-019-0257-1
36. Fernandez-Garcia, D., Hills, A., Page, K., Hastings, R.K., Toghill, B., Goddard, K.S. et al. (2019) Plasma cell-free DNA (cfDNA) as a predictive and prognostic marker in patients with metastatic breast cancer. Breast Cancer Res 21, 149. 10.1186/s13058-019-1235-8
37. Coombes, R.C., Page, K., Salari, R., Hastings, R.K., Armstrong, A., Ahmed, S. et al. (2019) Personalized Detection of Circulating Tumor DNA Antedates Breast Cancer Metastatic Recurrence. Clin Cancer Res 25, 4255-4263. 10.1158/1078-0432.CCR-18-3663
38. Wall, R.J., Ferguson, D.J.P., Freville, A., Franke-Fayard, B., Brady, D., Zeeshan, M. et al. (2018) Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis. Sci Rep 8, 5610. 10.1038/s41598-018-23871-9
39. Wall, R.J., Ferguson, D.J.P., Freville, A., Franke-Fayard, B., Brady, D., Zeeshan, M. et al. (2018) Author Correction: Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis. Sci Rep 8, 12814. 10.1038/s41598-018-30924-6
40. Myint, N.N.M., Verma, A.M., Fernandez-Garcia, D., Sarmah, P., Tarpey, P.S., Al-Aqbi, S.S. et al. (2018) Circulating tumor DNA in patients with colorectal adenomas: assessment of detectability and genetic heterogeneity. Cell Death Dis 9, 894. 10.1038/s41419-018-0934-x
41. Guttery, D.S., Hancox, R.A., Mulligan, K.T., Hughes, S., Lambe, S.M., Howard Pringle, J. et al. (2018) Correction to: Association of invasion-promoting tenascin-C additional domains with breast cancers in young women. Breast Cancer Res 20, 80. 10.1186/s13058-018-0997-8
42. Guttery, D.S. and Blighe, K. and Polymeros, K. and Symonds, R.P. and Macip, S. and Moss, E.L. (2018) Racial differences in endometrial cancer molecular portraits in The Cancer Genome Atlas. Oncotarget 9, 17093-17103. 10.18632/oncotarget.24907
43. Shaw, J.A., Guttery, D.S., Hills, A., Fernandez-Garcia, D., Page, K., Rosales, B.M. et al. (2017) Mutation Analysis of Cell-Free DNA and Single Circulating Tumor Cells in Metastatic Breast Cancer Patients with High Circulating Tumor Cell Counts. Clin Cancer Res 23, 88-96. 10.1158/1078-0432.CCR-16-0825
44. Saini, E., Zeeshan, M., Brady, D., Pandey, R., Kaiser, G., Koreny, L. et al. (2017) Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development. Sci Rep 7, 15577. 10.1038/s41598-017-15781-z
45. Page, K., Guttery, D.S., Fernandez-Garcia, D., Hills, A., Hastings, R.K., Luo, J. et al. (2017) Next Generation Sequencing of Circulating Cell-Free DNA for Evaluating Mutations and Gene Amplification in Metastatic Breast Cancer. Clin Chem 63, 532-541. 10.1373/clinchem.2016.261834
46. Openshaw, M.R. and Richards, C.J. and Guttery, D.S. and Shaw, J.A. and Thomas, A.L. (2017) The genetics of gastroesophageal adenocarcinoma and the use of circulating cell free DNA for disease detection and monitoring. Expert Rev Mol Diagn 17, 459-470. 10.1080/14737159.2017.1308824
47. Openshaw, M.R. and Page, K. and Fernandez-Garcia, D. and Guttery, D. and Shaw, J.A. (2016) The role of ctDNA detection and the potential of the liquid biopsy for breast cancer monitoring. Expert Rev Mol Diagn 16, 751-755. 10.1080/14737159.2016.1184974
48. Roques, M., Wall, R.J., Douglass, A.P., Ramaprasad, A., Ferguson, D.J., Kaindama, M.L. et al. (2015) Plasmodium P-Type Cyclin CYC3 Modulates Endomitotic Growth during Oocyst Development in Mosquitoes. PLoS Pathog 11, e1005273. 10.1371/journal.ppat.1005273
49. Magnani, L., Patten, D.K., Nguyen, V.T., Hong, S.P., Steel, J.H., Patel, N. et al. (2015) The pioneer factor PBX1 is a novel driver of metastatic progression in ERalpha-positive breast cancer. Oncotarget 6, 21878-21891. 10.18632/oncotarget.4243
50. Guttery, D.S. and Roques, M. and Holder, A.A. and Tewari, R. (2015) Commit and Transmit: Molecular Players in Plasmodium Sexual Development and Zygote Differentiation. Trends Parasitol 31, 676-685. 10.1016/j.pt.2015.08.002
51. Guttery, D.S., Page, K., Hills, A., Woodley, L., Marchese, S.D., Rghebi, B. et al. (2015) Noninvasive detection of activating estrogen receptor 1 (ESR1) mutations in estrogen receptor-positive metastatic breast cancer. Clin Chem 61, 974-982. 10.1373/clinchem.2015.238717
52. Palmieri, C., Cleator, S., Kilburn, L.S., Kim, S.B., Ahn, S.H., Beresford, M. et al. (2014) NEOCENT: a randomised feasibility and translational study comparing neoadjuvant endocrine therapy with chemotherapy in ER-rich postmenopausal primary breast cancer. Breast Cancer Res Treat 148, 581-590. 10.1007/s10549-014-3183-4
53. Guttery, D.S., Poulin, B., Ramaprasad, A., Wall, R.J., Ferguson, D.J., Brady, D. et al. (2014) Genome-wide functional analysis of Plasmodium protein phosphatases reveals key regulators of parasite development and differentiation. Cell Host Microbe 16, 128-140. 10.1016/j.chom.2014.05.020
54. Blighe, K., Kenny, L., Patel, N., Guttery, D.S., Page, K., Gronau, J.H. et al. (2014) Whole genome sequence analysis suggests intratumoral heterogeneity in dissemination of breast cancer to lymph nodes. PLoS One 9, e115346. 10.1371/journal.pone.0115346
55. Poulin, B., Patzewitz, E.M., Brady, D., Silvie, O., Wright, M.H., Ferguson, D.J. et al. (2013) Unique apicomplexan IMC sub-compartment proteins are early markers for apical polarity in the malaria parasite. Biol Open 2, 1160-1170. 10.1242/bio.20136163
56. Patzewitz, E.M., Guttery, D.S., Poulin, B., Ramakrishnan, C., Ferguson, D.J., Wall, R.J. et al. (2013) An ancient protein phosphatase, SHLP1, is critical to microneme development in Plasmodium ookinetes and parasite transmission. Cell Rep 3, 622-629. 10.1016/j.celrep.2013.01.032
57. Page, K., Guttery, D.S., Zahra, N., Primrose, L., Elshaw, S.R., Pringle, J.H. et al. (2013) Influence of plasma processing on recovery and analysis of circulating nucleic acids. PLoS One 8, e77963. 10.1371/journal.pone.0077963
58. Guttery, D.S., Pittman, J.K., Frenal, K., Poulin, B., McFarlane, L.R., Slavic, K. et al. (2013) The Plasmodium berghei Ca(2+)/H(+) exchanger, PbCAX, is essential for tolerance to environmental Ca(2+) during sexual development. PLoS Pathog 9, e1003191. 10.1371/journal.ppat.1003191
59. Guttery, D.S., Blighe, K., Page, K., Marchese, S.D., Hills, A., Coombes, R.C. et al. (2013) Hide and seek: tell-tale signs of breast cancer lurking in the blood. Cancer Metastasis Rev 32, 289-302. 10.1007/s10555-012-9414-4
60. Shaw, J.A., Page, K., Blighe, K., Hava, N., Guttery, D., Ward, B. et al. (2012) Genomic analysis of circulating cell-free DNA infers breast cancer dormancy. Genome Res 22, 220-231. 10.1101/gr.123497.111
61. Payne, R.E., Hava, N.L., Page, K., Blighe, K., Ward, B., Slade, M. et al. (2012) The presence of disseminated tumour cells in the bone marrow is inversely related to circulating free DNA in plasma in breast cancer dormancy. Br J Cancer 106, 375-382. 10.1038/bjc.2011.537
62. Guttery, D.S., Poulin, B., Ferguson, D.J., Szoor, B., Wickstead, B., Carroll, P.L. et al. (2012) A unique protein phosphatase with kelch-like domains (PPKL) in Plasmodium modulates ookinete differentiation, motility and invasion. PLoS Pathog 8, e1002948. 10.1371/journal.ppat.1002948
63. Guttery, D.S. and Holder, A.A. and Tewari, R. (2012) Sexual development in Plasmodium: lessons from functional analyses. PLoS Pathog 8, e1002404. 10.1371/journal.ppat.1002404
64. Guttery, D.S., Ferguson, D.J., Poulin, B., Xu, Z., Straschil, U., Klop, O. et al. (2012) A putative homologue of CDC20/CDH1 in the malaria parasite is essential for male gamete development. PLoS Pathog 8, e1002554. 10.1371/journal.ppat.1002554
65. Page, K., Hava, N., Ward, B., Brown, J., Guttery, D.S., Ruangpratheep, C. et al. (2011) Detection of HER2 amplification in circulating free DNA in patients with breast cancer. Br J Cancer 104, 1342-1348. 10.1038/bjc.2011.89
66. Guttery, D.S. and Shaw, J.A. and Lloyd, K. and Pringle, J.H. and Walker, R.A. (2010) Expression of tenascin-C and its isoforms in the breast. Cancer Metastasis Rev 29, 595-606. 10.1007/s10555-010-9249-9
67. Guttery, D.S., Hancox, R.A., Mulligan, K.T., Hughes, S., Lambe, S.M., Pringle, J.H. et al. (2010) Association of invasion-promoting tenascin-C additional domains with breast cancers in young women. Breast Cancer Res 12, R57. 10.1186/bcr2618
68. Russell, L.J., Capasso, M., Vater, I., Akasaka, T., Bernard, O.A., Calasanz, M.J. et al. (2009) Deregulated expression of cytokine receptor gene, CRLF2, is involved in lymphoid transformation in B-cell precursor acute lymphoblastic leukemia. Blood 114, 2688-2698. 10.1182/blood-2009-03-208397
69. Hancox, R.A., Allen, M.D., Holliday, D.L., Edwards, D.R., Pennington, C.J., Guttery, D.S. et al. (2009) Tumour-associated tenascin-C isoforms promote breast cancer cell invasion and growth by matrix metalloproteinase-dependent and independent mechanisms. Breast Cancer Res 11, R24. 10.1186/bcr2251
Supervision
Dr Guttery currently supervises PhD projects looking at the utility of ctDNA for earlier detection and monitoring of cancer, as well as joint projects with colleagues in the School of Informatics in improving diagnostic imaging using artificial intelligence.
Teaching
Dr Guttery is a course co-convenor for MSc Cancer Molecular Pathology and Therapeutics, and also teaches the following modules:
- BS3031 - Human Genetics
- BS3003 - Cancer Cell and Molecular Biology
- MB1080 - Introduction to Medical Sciences
Press and media
- Blood tests for cancer
- Artificial intelligence in earlier diagnosis
- Malaria
Activities
- Steering Committee Member - ECMC Junior Investigator Networking Group (JING)
- Higher Education Academy - Fellow
- Member - European Network for Individualised Treatment in Endometrial Cancer (ENITEC)
- Associate Editor - Oncogene