Zeiss MP7 multiphoton microscope
Currently out of use
- Maurice Shock Building, room 383.
- Two-photon absorption properties of fluorescent proteins (2011) M. Drobizhev, N.S. Makarov1, S.E. Tillo, T.E. Hughes and A. Rebane1. Nat. Meth. 8: 393-399.
- A new approach to dual-color two-photon microscopy with fluorescent proteins (2010) S.E. Tillo, T.E. Hughes, N.S. Makarov, A. Rebane, M. Drobizhev. BMC Biotechnol. 10: 6-12.
Multiphoton systems are used to image deeper into the sample. Using a normal confocal laser scanning microscope images can be taken up to 50 µm into the sample. This system should be able to image to a depth of around 1 mm.
This microscope is only for multiphoton imaging and is equipped with a Mai Tai eHP DS Ti:Sapphire pulsed laser (< 70 fs pulsewidth) with DeepSee dispersion. The laser is tunable between 690-1040nm.
The system is build around an upright Axio Examiner.Z1 microscope and has two non-descanned detectors (NDD) and a transmitted light detector.
One W-Plan-Apochromat 20x/NA=1 DIC(UV) VIS-IR water dipping objective is available. It is apochromatically corrected from VIS to IR and designed for use without cover slip.
- Mirror + SP485 nm
- Mirror + BP 455-500 nm
- Mirror + BP 500-550 nm
- LP490 nm + BP 500-550 nm
- LP510 nm + BP520-560 nm
- LP555 nm + BP 565-610 nm