Zeiss MP7 multiphoton microscope

Information 

Currently out of use

Location

• Maurice Shock Building, room 383.

Contact

• Mr Matthew Barker, +44 (0)116 252 3085
• Dr Kees Straatman, +44 (0)116 252 7085/2263

Literature

• Two-photon absorption properties of fluorescent proteins (2011) M. Drobizhev, N.S. Makarov1, S.E. Tillo, T.E. Hughes and A. Rebane1. Nat. Meth. 8: 393-399.
• A new approach to dual-color two-photon microscopy with fluorescent proteins (2010) S.E. Tillo, T.E. Hughes, N.S. Makarov, A. Rebane, M. Drobizhev. BMC Biotechnol. 10: 6-12.

Multiphoton systems are used to image deeper into the sample. Using a normal confocal laser scanning microscope images can be taken up to 50 µm into the sample. This system should be able to image to a depth of around 1 mm.

This microscope is only for multiphoton imaging and is equipped with a Mai Tai eHP DS Ti:Sapphire pulsed laser (< 70 fs pulsewidth) with DeepSee dispersion. The laser is tunable between 690-1040nm.

The system is build around an upright Axio Examiner.Z1 microscope and has two non-descanned detectors (NDD) and a transmitted light detector.

One W-Plan-Apochromat 20x/NA=1 DIC(UV) VIS-IR water dipping objective is available. It is apochromatically corrected from VIS to IR and designed for use without cover slip.

Filters

• Mirror + SP485 nm
• Mirror + BP 455-500 nm
• Mirror + BP 500-550 nm
• LP490 nm + BP 500-550 nm
• LP510 nm + BP520-560 nm
• LP555 nm + BP 565-610 nm